{"entity": "publication", "iuid": "94274bf8ef3f45f59b16382bef0510c6", "timestamp": "2026-09-28T11:18:01.957Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/94274bf8ef3f45f59b16382bef0510c6.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/94274bf8ef3f45f59b16382bef0510c6"}}, "title": "TcellSubC: An Atlas of the Subcellular Proteome of Human T Cells.", "authors": [{"family": "Joshi", "given": "Rubin Narayan", "initials": "RN"}, {"family": "Stadler", "given": "Charlotte", "initials": "C"}, {"family": "Lehmann", "given": "Robert", "initials": "R"}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J"}, {"family": "Tegn\u00e9r", "given": "Jesper", "initials": "J"}, {"family": "Schmidt", "given": "Angelika", "initials": "A"}, {"family": "Vesterlund", "given": "Mattias", "initials": "M"}], "type": "journal article", "published": "2019-11-26", "journal": {"title": "Front Immunol", "issn": "1664-3224", "volume": "10", "pages": "2708", "issn-l": "1664-3224"}, "abstract": "We have curated an in-depth subcellular proteomic map of primary human CD4+ T cells, divided into cytosolic, nuclear and membrane fractions generated by an optimized fractionation and HiRIEF-LC-MS/MS workflow for limited amounts of primary cells. The subcellular proteome of T cells was mapped under steady state conditions, as well as upon 15 min and 1 h of T cell receptor (TCR) stimulation, respectively. We quantified the subcellular distribution of 6,572 proteins and identified a subset of 237 potentially translocating proteins, including both well-known examples and novel ones. Microscopic validation confirmed the localization of selected proteins with previously known and unknown localization, respectively. We further provide the data in an easy-to-use web platform to facilitate re-use, as the data can be relevant for basic research as well as for clinical exploitation of T cells as therapeutic targets.", "doi": "10.3389/fimmu.2019.02708", "pmid": "31849937", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6902019"}], "notes": [], "created": "2026-09-23T11:52:24.370Z", "modified": "2026-09-23T11:52:24.394Z"}