{"entity": "publication", "iuid": "929a3bd6a28948ebb298a363a9b596c7", "timestamp": "2026-09-01T08:24:47.508Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/929a3bd6a28948ebb298a363a9b596c7.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/929a3bd6a28948ebb298a363a9b596c7"}}, "title": "Single-Cell and Spatial Transcriptomic Analysis of Human Skin Delineates Intercellular Communication and Pathogenic Cells.", "authors": [{"family": "Thrane", "given": "Kim", "initials": "K"}, {"family": "Winge", "given": "M\u00e5rten C G", "initials": "MCG"}, {"family": "Wang", "given": "Hongyu", "initials": "H"}, {"family": "Chen", "given": "Larry", "initials": "L"}, {"family": "Guo", "given": "Margaret G", "initials": "MG"}, {"family": "Andersson", "given": "Alma", "initials": "A"}, {"family": "Abalo", "given": "Xes\u00fas M", "initials": "XM"}, {"family": "Yang", "given": "Xue", "initials": "X"}, {"family": "Kim", "given": "Daniel S", "initials": "DS"}, {"family": "Longo", "given": "Sophia K", "initials": "SK"}, {"family": "Soong", "given": "Brian Y", "initials": "BY"}, {"family": "Meyers", "given": "Jordan M", "initials": "JM"}, {"family": "Reynolds", "given": "David L", "initials": "DL"}, {"family": "McGeever", "given": "Aaron", "initials": "A"}, {"family": "Demircioglu", "given": "Deniz", "initials": "D"}, {"family": "Hasson", "given": "Dan", "initials": "D"}, {"family": "Mirzazadeh", "given": "Reza", "initials": "R"}, {"family": "Rubin", "given": "Adam J", "initials": "AJ"}, {"family": "Bae", "given": "Gordon H", "initials": "GH"}, {"family": "Karkanias", "given": "Jim", "initials": "J"}, {"family": "Rieger", "given": "Kerri", "initials": "K"}, {"family": "Lundeberg", "given": "Joakim", "initials": "J"}, {"family": "Ji", "given": "Andrew L", "initials": "AL"}], "type": "journal article", "published": "2023-11-00", "journal": {"title": "J. Invest. Dermatol.", "issn": "1523-1747", "volume": "143", "issue": "11", "pages": "2177-2192.e13", "issn-l": "0022-202X"}, "abstract": "Epidermal homeostasis is governed by a balance between keratinocyte proliferation and differentiation with contributions from cell-cell interactions, but conserved or divergent mechanisms governing this equilibrium across species and how an imbalance contributes to skin disease are largely undefined. To address these questions, human skin single-cell RNA sequencing and spatial transcriptomics data were integrated and compared with mouse skin data. Human skin cell-type annotation was improved using matched spatial transcriptomics data, highlighting the importance of spatial context in cell-type identity, and spatial transcriptomics refined cellular communication inference. In cross-species analyses, we identified a human spinous keratinocyte subpopulation that exhibited proliferative capacity and a heavy metal processing signature, which was absent in mouse and may account for species differences in epidermal thickness. This human subpopulation was expanded in psoriasis and zinc-deficiency dermatitis, attesting to disease relevance and suggesting a paradigm of subpopulation dysfunction as a hallmark of the disease. To assess additional potential subpopulation drivers of skin diseases, we performed cell-of-origin enrichment analysis within genodermatoses, nominating pathogenic cell subpopulations and their communication pathways, which highlighted multiple potential therapeutic targets. This integrated dataset is encompassed in a publicly available web resource to aid mechanistic and translational studies of normal and diseased skin.", "doi": "10.1016/j.jid.2023.02.040", "pmid": "37142187", "labels": [], "xrefs": [{"db": "mid", "key": "NIHMS1898872"}, {"db": "pmc", "key": "PMC10592679"}, {"db": "pii", "key": "S0022-202X(23)02040-7"}], "notes": [], "created": "2026-08-20T07:59:24.676Z", "modified": "2026-08-20T07:59:24.730Z"}