{"entity": "publication", "iuid": "9029facacff8400cbee946dfce01cb57", "timestamp": "2026-09-28T11:16:13.116Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/9029facacff8400cbee946dfce01cb57.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/9029facacff8400cbee946dfce01cb57"}}, "title": "Myc-dependent endothelial proliferation is controlled by phosphotyrosine 1212 in VEGF receptor-2.", "authors": [{"family": "Testini", "given": "Chiara", "initials": "C"}, {"family": "Smith", "given": "Ross O", "initials": "RO"}, {"family": "Jin", "given": "Yi", "initials": "Y"}, {"family": "Martinsson", "given": "Pernilla", "initials": "P"}, {"family": "Sun", "given": "Ying", "initials": "Y"}, {"family": "Hedlund", "given": "Marie", "initials": "M"}, {"family": "S\u00e1inz-Jaspeado", "given": "Miguel", "initials": "M"}, {"family": "Shibuya", "given": "Masabumi", "initials": "M"}, {"family": "Hellstr\u00f6m", "given": "Mats", "initials": "M"}, {"family": "Claesson-Welsh", "given": "Lena", "initials": "L", "orcid": "0000-0003-4275-2000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b07fbf00dad4ac8a4a53402888d55ef.json"}}], "type": "journal article", "published": "2019-11-05", "journal": {"title": "EMBO Rep.", "issn": "1469-3178", "volume": "20", "issue": "11", "pages": "e47845", "issn-l": "1469-221X"}, "abstract": "Exaggerated signaling by vascular endothelial growth factor (VEGF)-A and its receptor, VEGFR2, in pathologies results in poor vessel function. Still, pharmacological suppression of VEGFA/VEGFR2 may aggravate disease. Delineating VEGFR2 signaling in vivo provides strategies for suppression of specific VEGFR2-induced pathways. Three VEGFR2 tyrosine residues (Y949, Y1212, and Y1173) induce downstream signaling. Here, we show that knock-in of phenylalanine to create VEGFR2 Y1212F in C57Bl/6 and FVB mouse strains leads to loss of growth factor receptor-bound protein 2- and phosphoinositide 3'-kinase (PI3K)p85 signaling. C57Bl/6 Vegfr2Y1212F/Y1212F show reduced embryonic endothelial cell (EC) proliferation and partial lethality. FVB Vegfr2Y1212F/Y1212F show reduced postnatal EC proliferation. Reduced EC proliferation in Vegfr2Y1212F/Y1212F explants is rescued by c-Myc overexpression. We conclude that VEGFR2 Y1212 signaling induces activation of extracellular-signal-regulated kinase (ERK)1/2 and Akt pathways required for c-Myc-dependent gene regulation, endothelial proliferation, and vessel stability.", "doi": "10.15252/embr.201947845", "pmid": "31545012", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6832004"}, {"db": "GEO", "key": "GSE136085"}], "notes": [], "created": "2026-09-23T09:44:22.908Z", "modified": "2026-09-23T10:18:39.189Z"}