{"entity": "publication", "iuid": "89f9b2c01cbd487cbf38391bc959dbad", "timestamp": "2026-08-20T20:45:50.270Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/89f9b2c01cbd487cbf38391bc959dbad.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/89f9b2c01cbd487cbf38391bc959dbad"}}, "title": "3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures.", "authors": [{"family": "Khati", "given": "Vamakshi", "initials": "V"}, {"family": "Ramachandraiah", "given": "Harisha", "initials": "H"}, {"family": "Pati", "given": "Falguni", "initials": "F", "orcid": "0000-0002-3588-1800", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a980f4446de44c9ca89931a1c774f1e6.json"}}, {"family": "Svahn", "given": "Helene A", "initials": "HA"}, {"family": "Gaudenzi", "given": "Giulia", "initials": "G"}, {"family": "Russom", "given": "Aman", "initials": "A"}], "type": "journal article", "published": "2022-07-13", "journal": {"title": "Biosensors (Basel)", "issn": "2079-6374", "volume": "12", "issue": "7", "issn-l": null}, "abstract": "Bioprinting is an acclaimed technique that allows the scaling of 3D architectures in an organized pattern but suffers from a scarcity of appropriate bioinks. Decellularized extracellular matrix (dECM) from xenogeneic species has garnered support as a biomaterial to promote tissue-specific regeneration and repair. The prospect of developing dECM-based 3D artificial tissue is impeded by its inherent low mechanical properties. In recent years, 3D bioprinting of dECM-based bioinks modified with additional scaffolds has advanced the development of load-bearing constructs. However, previous attempts using dECM were limited to low-temperature bioprinting, which is not favorable for a longer print duration with cells. Here, we report the development of a multi-material decellularized liver matrix (dLM) bioink reinforced with gelatin and polyethylene glycol to improve rheology, extrudability, and mechanical stability. This shear-thinning bioink facilitated extrusion-based bioprinting at 37 \u00b0C with HepG2 cells into a 3D grid structure with a further enhancement for long-term applications by enzymatic crosslinking with mushroom tyrosinase. The heavily crosslinked structure showed a 16-fold increase in viscosity (2.73 Pa s-1) and a 32-fold increase in storage modulus from the non-crosslinked dLM while retaining high cell viability (85-93%) and liver-specific functions. Our results show that the cytocompatible crosslinking of dLM bioink at physiological temperatures has promising applications for extended 3D-printing procedures.", "doi": "10.3390/bios12070521", "pmid": "35884324", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9313433"}, {"db": "pii", "key": "bios12070521"}], "notes": [], "created": "2026-08-20T13:38:58.648Z", "modified": "2026-08-20T13:38:58.770Z"}