{"entity": "publication", "iuid": "8579de5710e642238f0820ba8c1f0974", "timestamp": "2026-11-11T18:25:31.210Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/8579de5710e642238f0820ba8c1f0974.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/8579de5710e642238f0820ba8c1f0974"}}, "title": "CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes.", "authors": [{"family": "Gupta", "given": "Meghna", "initials": "M"}, {"family": "Azumaya", "given": "Caleigh M", "initials": "CM"}, {"family": "Moritz", "given": "Michelle", "initials": "M"}, {"family": "Pourmal", "given": "Sergei", "initials": "S"}, {"family": "Diallo", "given": "Amy", "initials": "A"}, {"family": "Merz", "given": "Gregory E", "initials": "GE"}, {"family": "Jang", "given": "Gwendolyn", "initials": "G"}, {"family": "Bouhaddou", "given": "Mehdi", "initials": "M"}, {"family": "Fossati", "given": "Andrea", "initials": "A"}, {"family": "Brilot", "given": "Axel F", "initials": "AF"}, {"family": "Diwanji", "given": "Devan", "initials": "D"}, {"family": "Hernandez", "given": "Evelyn", "initials": "E"}, {"family": "Herrera", "given": "Nadia", "initials": "N"}, {"family": "Kratochvil", "given": "Huong T", "initials": "HT"}, {"family": "Lam", "given": "Victor L", "initials": "VL"}, {"family": "Li", "given": "Fei", "initials": "F"}, {"family": "Li", "given": "Yang", "initials": "Y"}, {"family": "Nguyen", "given": "Henry C", "initials": "HC"}, {"family": "Nowotny", "given": "Carlos", "initials": "C"}, {"family": "Owens", "given": "Tristan W", "initials": "TW"}, {"family": "Peters", "given": "Jessica K", "initials": "JK"}, {"family": "Rizo", "given": "Alexandrea N", "initials": "AN"}, {"family": "Schulze-Gahmen", "given": "Ursula", "initials": "U"}, {"family": "Smith", "given": "Amber M", "initials": "AM"}, {"family": "Young", "given": "Iris D", "initials": "ID"}, {"family": "Yu", "given": "Zanlin", "initials": "Z"}, {"family": "Asarnow", "given": "Daniel", "initials": "D"}, {"family": "Billesb\u00f8lle", "given": "Christian", "initials": "C"}, {"family": "Campbell", "given": "Melody G", "initials": "MG"}, {"family": "Chen", "given": "Jen", "initials": "J"}, {"family": "Chen", "given": "Kuei-Ho", "initials": "KH"}, {"family": "Chio", "given": "Un Seng", "initials": "US"}, {"family": "Dickinson", "given": "Miles Sasha", "initials": "MS"}, {"family": "Doan", "given": "Loan", "initials": "L"}, {"family": "Jin", "given": "Mingliang", "initials": "M"}, {"family": "Kim", "given": "Kate", "initials": "K"}, {"family": "Li", "given": "Junrui", "initials": "J"}, {"family": "Li", "given": "Yen-Li", "initials": "YL"}, {"family": "Linossi", "given": "Edmond", "initials": "E"}, {"family": "Liu", "given": "Yanxin", "initials": "Y"}, {"family": "Lo", "given": "Megan", "initials": "M"}, {"family": "Lopez", "given": "Jocelyne", "initials": "J"}, {"family": "Lopez", "given": "Kyle E", "initials": "KE"}, {"family": "Mancino", "given": "Adamo", "initials": "A"}, {"family": "Moss", "given": "Frank R", "initials": "FR"}, {"family": "Paul", "given": "Michael D", "initials": "MD"}, {"family": "Pawar", "given": "Komal Ishwar", "initials": "KI"}, {"family": "Pelin", "given": "Adrian", "initials": "A"}, {"family": "Pospiech", "given": "Thomas H", "initials": "TH"}, {"family": "Puchades", "given": "Cristina", "initials": "C"}, {"family": "Remesh", "given": "Soumya Govinda", "initials": "SG"}, {"family": "Safari", "given": "Maliheh", "initials": "M"}, {"family": "Schaefer", "given": "Kaitlin", "initials": "K"}, {"family": "Sun", "given": "Ming", "initials": "M"}, {"family": "Tabios", "given": "Mariano C", "initials": "MC"}, {"family": "Thwin", "given": "Aye C", "initials": "AC"}, {"family": "Titus", "given": "Erron W", "initials": "EW"}, {"family": "Trenker", "given": "Raphael", "initials": "R"}, {"family": "Tse", "given": "Eric", "initials": "E"}, {"family": "Tsui", "given": "Tsz Kin Martin", "initials": "TKM"}, {"family": "Wang", "given": "Feng", "initials": "F"}, {"family": "Zhang", "given": "Kaihua", "initials": "K"}, {"family": "Zhang", "given": "Yang", "initials": "Y"}, {"family": "Zhao", "given": "Jianhua", "initials": "J"}, {"family": "Zhou", "given": "Fengbo", "initials": "F"}, {"family": "Zhou", "given": "Yuan", "initials": "Y"}, {"family": "Zuliani-Alvarez", "given": "Lorena", "initials": "L"}, {"family": "QCRG Structural Biology Consortium", "given": "", "initials": ""}, {"family": "Agard", "given": "David A", "initials": "DA"}, {"family": "Cheng", "given": "Yifan", "initials": "Y"}, {"family": "Fraser", "given": "James S", "initials": "JS"}, {"family": "Jura", "given": "Natalia", "initials": "N"}, {"family": "Kortemme", "given": "Tanja", "initials": "T"}, {"family": "Manglik", "given": "Aashish", "initials": "A"}, {"family": "Southworth", "given": "Daniel R", "initials": "DR"}, {"family": "Stroud", "given": "Robert M", "initials": "RM"}, {"family": "Swaney", "given": "Danielle L", "initials": "DL"}, {"family": "Krogan", "given": "Nevan J", "initials": "NJ"}, {"family": "Frost", "given": "Adam", "initials": "A"}, {"family": "Rosenberg", "given": "Oren S", "initials": "OS"}, {"family": "Verba", "given": "Kliment A", "initials": "KA"}], "type": "preprint", "published": "2021-05-11", "journal": {"title": "bioRxiv", "issn": "2692-8205", "issn-l": null}, "abstract": "The SARS-CoV-2 protein Nsp2 has been implicated in a wide range of viral processes, but its exact functions, and the structural basis of those functions, remain unknown. Here, we report an atomic model for full-length Nsp2 obtained by combining cryo-electron microscopy with deep learning-based structure prediction from AlphaFold2. The resulting structure reveals a highly-conserved zinc ion-binding site, suggesting a role for Nsp2 in RNA binding. Mapping emerging mutations from variants of SARS-CoV-2 on the resulting structure shows potential host-Nsp2 interaction regions. Using structural analysis together with affinity tagged purification mass spectrometry experiments, we identify Nsp2 mutants that are unable to interact with the actin-nucleation-promoting WASH protein complex or with GIGYF2, an inhibitor of translation initiation and modulator of ribosome-associated quality control. Our work suggests a potential role of Nsp2 in linking viral transcription within the viral replication-transcription complexes (RTC) to the translation initiation of the viral message. Collectively, the structure reported here, combined with mutant interaction mapping, provides a foundation for functional studies of this evolutionary conserved coronavirus protein and may assist future drug design.", "doi": "10.1101/2021.05.10.443524", "pmid": "34013269", "labels": {"Andrea Fossati": null, "DDLS Fellow": null}, "xrefs": [{"db": "pmc", "key": "PMC8132225"}, {"db": "pii", "key": "2021.05.10.443524"}], "notes": [], "created": "2026-11-06T08:03:51.117Z", "modified": "2026-11-06T08:03:51.145Z"}