{"entity": "publication", "iuid": "81169b717ab24fff95362ee17614b148", "timestamp": "2026-08-29T04:18:13.130Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/81169b717ab24fff95362ee17614b148.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/81169b717ab24fff95362ee17614b148"}}, "title": "Lab-on-PCB with integrated DNA amplification and electroanalytical detection for point-of-care diagnostics.", "authors": [{"family": "Thorapalli Muralidharan", "given": "Seshagopalan", "initials": "S"}, {"family": "Hanze", "given": "Martin", "initials": "M"}, {"family": "Ainla", "given": "Alar", "initials": "A"}, {"family": "M\u00f6ller", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Hamedi", "given": "Mahiar Max", "initials": "MM"}, {"family": "Toldr\u00e0", "given": "Anna", "initials": "A"}], "type": "journal article", "published": "2025-09-12", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "15", "issue": "1", "pages": "32418", "issn-l": "2045-2322"}, "abstract": "Nucleic acid amplification tests (NAATs) are powerful medical diagnostic tools for point-of-care (POC) and other field applications. However, traditional methods like quantitative PCR (qPCR) require complex, expensive equipment and trained operators, limiting their use to centralized labs. Isothermal alternatives, like Loop-mediated Isothermal Amplification (LAMP), are better adapted for POC devices. Lab-on-PCB systems have the potential to overcome the challenges faced by conventional microfabrication-based systems. This study presents a novel lab-on-PCB device for nucleic acid amplification and electrochemical detection using reverse transcription LAMP (RT-LAMP) of SARS-CoV-2. The system consists of two disposable PCB-based chips making it close to zero cost. One PCB is for heating and nucleic acid amplification, while the other is for electrochemical detection using Cyclic Voltammetry (CV) with a redox-active intercalator. The PCB slides are connected to a compact electronic device (< 10 USD) for controlling the heating and electroanalytical readout. Using this device, we achieved successful rapid (< 1.5 h) nucleic acid amplification and detection at a target concentration of 10 copies/reaction. This work represents a notable step toward developing integrated, portable NAAT devices for POC diagnostics.", "doi": "10.1038/s41598-025-12364-1", "pmid": "40940334", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12432110"}, {"db": "pii", "key": "10.1038/s41598-025-12364-1"}], "notes": [], "created": "2026-08-21T11:54:16.124Z", "modified": "2026-08-21T11:54:16.136Z"}