{"entity": "publication", "iuid": "7f0fc73121214aecafb2fc59b2247484", "timestamp": "2026-08-20T20:42:22.782Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7f0fc73121214aecafb2fc59b2247484.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7f0fc73121214aecafb2fc59b2247484"}}, "title": "A simplified approach to control cell adherence on biologically derived in vitro cell culture scaffolds by direct UV-mediated RGD linkage.", "authors": [{"family": "Porras Hern\u00e1ndez", "given": "A M", "initials": "AM", "orcid": "0000-0001-5952-2418", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3c7742b33f8499baad542f0a2738a27.json"}}, {"family": "Pohlit", "given": "H", "initials": "H"}, {"family": "Sj\u00f6gren", "given": "F", "initials": "F"}, {"family": "Shi", "given": "L", "initials": "L"}, {"family": "Ossipov", "given": "D", "initials": "D"}, {"family": "Antfolk", "given": "M", "initials": "M"}, {"family": "Tenje", "given": "M", "initials": "M"}], "type": "journal article", "published": "2020-10-14", "journal": {"title": "J Mater Sci Mater Med", "issn": "1573-4838", "volume": "31", "issue": "10", "pages": "89", "issn-l": "0957-4530"}, "abstract": "In this work, we present a method to fabricate a hyaluronic acid (HA) hydrogel with spatially controlled cell-adhesion properties based on photo-polymerisation cross-linking and functionalization. The approach utilises the same reaction pathway for both steps meaning that it is user-friendly and allows for adaptation at any stage during the fabrication process. Moreover, the process does not require any additional cross-linkers. The hydrogel is formed by UV-initiated radical addition reaction between acrylamide (Am) groups on the HA backbone. Cell adhesion is modulated by functionalising the adhesion peptide sequence arginine-glycine-aspartate onto the hydrogel surface via radical mediated thiol-ene reaction using the non-reacted Am groups. We show that 10 \u00d7 10 \u00b5m2 squares could be patterned with sharp features and a good resolution. The smallest area that could be patterned resulting in good cell adhesion was 25 \u00d7 25 \u00b5m2 squares, showing single-cell adhesion. Mouse brain endothelial cells adhered and remained in culture for up to 7 days on 100 \u00d7 100 \u00b5m2 square patterns. We see potential for this material combination for future use in novel organ-on-chip models and tissue engineering where the location of the cells is of importance and to further study endothelial cell biology.", "doi": "10.1007/s10856-020-06446-x", "pmid": "33057798", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7560931"}, {"db": "pii", "key": "10.1007/s10856-020-06446-x"}], "notes": [], "created": "2026-08-20T06:40:01.694Z", "modified": "2026-08-20T06:40:01.786Z"}