{"entity": "publication", "iuid": "7e03c6cce00b4f8e83fb97029dcc2cbf", "timestamp": "2026-09-25T23:01:32.643Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7e03c6cce00b4f8e83fb97029dcc2cbf.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7e03c6cce00b4f8e83fb97029dcc2cbf"}}, "title": "Multifaceted Proteome Analysis at Solubility, Redox, and Expression Dimensions for Target Identification.", "authors": [{"family": "Saei", "given": "Amir A", "initials": "AA"}, {"family": "Lundin", "given": "Albin", "initials": "A"}, {"family": "Lyu", "given": "Hezheng", "initials": "H"}, {"family": "Gharibi", "given": "Hassan", "initials": "H"}, {"family": "Luo", "given": "Huqiao", "initials": "H"}, {"family": "Teppo", "given": "Jaakko", "initials": "J"}, {"family": "Zhang", "given": "Xuepei", "initials": "X"}, {"family": "Gaetani", "given": "Massimiliano", "initials": "M"}, {"family": "V\u00e9gv\u00e1ri", "given": "\u00c1kos", "initials": "\u00c1"}, {"family": "Holmdahl", "given": "Rikard", "initials": "R"}, {"family": "Gygi", "given": "Steven P", "initials": "SP"}, {"family": "Zubarev", "given": "Roman A", "initials": "RA", "orcid": "0000-0001-9839-2089", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5e3f9910c1ff434c8056bdecf537e9ef.json"}}], "type": "journal article", "published": "2024-10-00", "journal": {"title": "Adv Sci (Weinh)", "issn": "2198-3844", "volume": "11", "issue": "38", "pages": "e2401502", "issn-l": null}, "abstract": "Multifaceted interrogation of the proteome deepens the system-wide understanding of biological systems; however, mapping the redox changes in the proteome has so far been significantly more challenging than expression and solubility/stability analyses. Here, the first high-throughput redox proteomics approach integrated with expression analysis (REX) is devised and combined with the Proteome Integral Solubility Alteration (PISA) assay. The whole PISA-REX experiment with up to four biological replicates can be multiplexed into a single tandem mass tag TMTpro set. For benchmarking this compact tool, HCT116 cells treated with auranofin are analyzed, showing great improvement compared with previous studies. PISA-REX is then applied to study proteome remodeling upon stimulation of human monocytes by interferon \u03b1 (IFN-\u03b1). Applying this tool to study the proteome changes in plasmacytoid dendritic cells (pDCs) isolated from wild-type versus Ncf1-mutant mice treated with interferon \u03b1, shows that NCF1 deficiency enhances the STAT1 pathway and modulates the expression, solubility, and redox state of interferon-induced proteins. Providing comprehensive multifaceted information on the proteome, the compact PISA-REX has the potential to become an industry standard in proteomics and to open new windows into the biology of health and disease.", "doi": "10.1002/advs.202401502", "pmid": "39120068", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11481203"}], "notes": [], "created": "2026-09-23T11:16:49.839Z", "modified": "2026-09-23T11:16:49.911Z"}