{"entity": "publication", "iuid": "7cba108f903e4a5692805ab5d34e2222", "timestamp": "2026-09-25T15:28:14.184Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7cba108f903e4a5692805ab5d34e2222.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7cba108f903e4a5692805ab5d34e2222"}}, "title": "Massively parallel analysis of single-molecule dynamics on next-generation sequencing chips.", "authors": [{"family": "Aguirre Rivera", "given": "J", "initials": "J", "orcid": "0000-0002-5263-3577", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/07e78e63794e418ba37c40cec62dab87.json"}}, {"family": "Mao", "given": "G", "initials": "G", "orcid": "0000-0001-5841-0991", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ff1fec9d88414562b497c4387b256b2a.json"}}, {"family": "Sabantsev", "given": "A", "initials": "A", "orcid": "0000-0002-8559-8894", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1a997c393bf2489699c700ddceecb885.json"}}, {"family": "Panfilov", "given": "M", "initials": "M", "orcid": "0000-0003-2875-7315", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/757c08197cec42ccb3995c0af1bb79ca.json"}}, {"family": "Hou", "given": "Q", "initials": "Q", "orcid": "0009-0007-9296-4788", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b5dbff809dc34967b65c92ebc84795e6.json"}}, {"family": "Lindell", "given": "M", "initials": "M"}, {"family": "Chanez", "given": "C", "initials": "C", "orcid": "0000-0002-3432-4375", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8ad775c42e0f4a0da033804ce844ec7f.json"}}, {"family": "Ritort", "given": "F", "initials": "F", "orcid": "0000-0002-4869-5003", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b00fbaf455a34f1cbb24f79f80921a31.json"}}, {"family": "Jinek", "given": "M", "initials": "M", "orcid": "0000-0002-7601-210X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d31b60956cef4180bb0cdd762a49a638.json"}}, {"family": "Deindl", "given": "S", "initials": "S", "orcid": "0000-0001-6807-8654", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d409ebe1ddd945279b82704b1942fa93.json"}}], "type": "journal article", "published": "2024-08-23", "journal": {"title": "Science (New York, N.Y.)", "issn": "1095-9203", "volume": "385", "issue": "6711", "pages": "892-898", "issn-l": "0036-8075"}, "abstract": "Single-molecule techniques are ideally poised to characterize complex dynamics but are typically limited to investigating a small number of different samples. However, a large sequence or chemical space often needs to be explored to derive a comprehensive understanding of complex biological processes. Here we describe multiplexed single-molecule characterization at the library scale (MUSCLE), a method that combines single-molecule fluorescence microscopy with next-generation sequencing to enable highly multiplexed observations of complex dynamics. We comprehensively profiled the sequence dependence of DNA hairpin properties and Cas9-induced target DNA unwinding-rewinding dynamics. The ability to explore a large sequence space for Cas9 allowed us to identify a number of target sequences with unexpected behaviors. We envision that MUSCLE will enable the mechanistic exploration of many fundamental biological processes.", "doi": "10.1126/science.adn5371", "pmid": "39172826", "labels": {"SciLifeLab Fellow": "", "Sebastian Deindl": ""}, "xrefs": [], "notes": [], "created": "2026-09-23T08:53:53.800Z", "modified": "2026-09-23T08:53:54.025Z"}