{"entity": "publication", "iuid": "7a6438a6fb714da0b5fe46df56f2b29d", "timestamp": "2026-10-01T13:06:49.563Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7a6438a6fb714da0b5fe46df56f2b29d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7a6438a6fb714da0b5fe46df56f2b29d"}}, "title": "Quantification of proteomic profile changes in the hemolymph of crayfish during in vitro coagulation.", "authors": [{"family": "Mengal", "given": "Kifayatullah", "initials": "K"}, {"family": "Kor", "given": "Golara", "initials": "G"}, {"family": "Siino", "given": "Valentina", "initials": "V"}, {"family": "Bu\u0159i\u010d", "given": "Milo\u0161", "initials": "M"}, {"family": "Koz\u00e1k", "given": "Pavel", "initials": "P"}, {"family": "Levander", "given": "Fredrik", "initials": "F"}, {"family": "Niksirat", "given": "Hamid", "initials": "H"}], "type": "journal article", "published": "2023-10-00", "journal": {"title": "Dev. Comp. Immunol.", "issn": "1879-0089", "volume": "147", "pages": "104760", "issn-l": "0145-305X"}, "abstract": "Hemolymph is the circulatory fluid that fills the body cavity of crustaceans, analogous to blood in vertebrates. Hemolymph coagulation, similar to blood clotting in vertebrates, plays a crucial role in wound healing and innate immune responses. Despite extensive studies on the clotting process in crustaceans, no comparative quantitative analysis of the protein composition of non-clotted and clotted hemolymph in any decapod has been reported. In this study, we used label-free protein quantification with high-resolution mass spectrometry to identify the proteomic profile of hemolymph in crayfish and quantify significant changes in protein abundances between non-clotted and clotted hemolymph. Our analysis identified a total of two-hundred and nineteen proteins in both hemolymph groups. Furthermore, we discussed the potential functions of the top most high and low-abundant proteins in hemolymph proteomic profile. The quantity of most of the proteins was not significantly changed during coagulation between non-clotted and clotted hemolymph, which may indicate that clotting proteins are likely pre-synthesized, allowing for a swift coagulation response to injury. Four proteins still showed abundance differences (p < 0.05, fold change>2), including C-type lectin domain-containing proteins, Laminin A chain, Tropomyosin, and Reverse transcriptase domain-containing proteins. While the first three proteins were down-regulated, the last one was up-regulated. The down-regulation of structural and cytoskeletal proteins may affect the process of hemocyte degranulation needed for coagulation, while the up-regulation of an immune-related protein might be attributed to the phagocytosis ability of viable hemocytes during coagulation.", "doi": "10.1016/j.dci.2023.104760", "pmid": "37331675", "labels": [], "xrefs": [{"db": "pii", "key": "S0145-305X(23)00130-1"}], "notes": [], "created": "2026-09-23T12:14:13.592Z", "modified": "2026-09-23T12:14:13.607Z"}