{"entity": "publication", "iuid": "7542ff3807ec49a9b699bbcfafc498e0", "timestamp": "2026-08-26T22:47:02.913Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7542ff3807ec49a9b699bbcfafc498e0.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7542ff3807ec49a9b699bbcfafc498e0"}}, "title": "Generation of a human Neurochondrin deficient iPSC line KICRi002-A-3 using CRISPR/Cas9.", "authors": [{"family": "Fatima", "given": "Ambrin", "initials": "A"}, {"family": "Schuster", "given": "Jens", "initials": "J"}, {"family": "Akram", "given": "Talia", "initials": "T"}, {"family": "Sobol", "given": "Maria", "initials": "M"}, {"family": "Hoeber", "given": "Jan", "initials": "J"}, {"family": "Dahl", "given": "Niklas", "initials": "N"}], "type": "journal article", "published": "2020-04-00", "journal": {"title": "Stem Cell Res", "issn": "1876-7753", "volume": "44", "pages": "101758", "issn-l": "1873-5061"}, "abstract": "The role of Neurochondrin (NCDN) in humans is not well understood. Mice with a conditional Ncdn knock-out show epileptic seizures, depressive-like behaviours and impaired spatial learning. Using CRISPR/Cas9, we generated a Neurochondrin deficient human iPSC line KICRi002-A-3 carrying a homozygous 752 bp deletion / 2 bp insertion in the NCDN gene. The iPSC line maintained a normal 46,XY karyotype, expressed pluripotency markers and exhibited capability to differentiate into the three germ layers in vitro. Off-target editing was excluded and Neurochondrin expression was not detectable. The iPSC line offers a valuable resource to study the role of Neurochondrin during human neurogenesis.", "doi": "10.1016/j.scr.2020.101758", "pmid": "32203915", "labels": [], "xrefs": [{"db": "pii", "key": "S1873-5061(20)30062-3"}], "notes": [], "created": "2026-08-21T11:28:44.495Z", "modified": "2026-08-21T11:28:44.520Z"}