{"entity": "publication", "iuid": "725a6e50bdad4720b59f9642fb69d9c5", "timestamp": "2026-08-20T20:36:41.549Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/725a6e50bdad4720b59f9642fb69d9c5.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/725a6e50bdad4720b59f9642fb69d9c5"}}, "title": "In Vitro Elimination of Highly Multidrug-Resistant Bacteria by the Lactic Acid Bacterial Drug Candidate ILP100.", "authors": [{"family": "Lofton-Tomenius", "given": "Hava", "initials": "H"}, {"family": "Pang", "given": "Yanhong", "initials": "Y"}, {"family": "Pallin", "given": "Anton", "initials": "A"}, {"family": "Myktybekova", "given": "Zhanar", "initials": "Z"}, {"family": "Lelham", "given": "Ninus", "initials": "N"}, {"family": "Riesbeck", "given": "Kristian", "initials": "K"}, {"family": "V\u00e5gesj\u00f6", "given": "Evelina", "initials": "E"}, {"family": "Roos", "given": "Stefan", "initials": "S"}, {"family": "Phillipson", "given": "Mia", "initials": "M", "orcid": "0000-0002-2387-0266", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/265c9b3e11de4fcaa270a0eb20faf65c.json"}}], "type": "journal article", "published": "2025-05-00", "journal": {"title": "Infect Dis Ther", "issn": "2193-8229", "volume": "14", "issue": "5", "pages": "1119-1131", "issn-l": null}, "abstract": "Multidrug resistance (MDR) has been identified in wound bacterial isolates from Ukrainian war victims treated in Ukraine and across Europe. ILP100, a drug candidate for the treatment of skin wounds, is composed of a Limosilactobacillus reuteri expressing human chemokine CXCL12. In this study, the antimicrobial effects of ILP100 were tested on MDR bacteria isolated from wounds of Ukrainian war victims.\n\nILP100 was co-cultured with one of the wound pathogens (Pseudomonas aeruginosa, Acinetobacter baumannii, Enterobacter cloacae, Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus; 12 non-MDR and 12 MDR isolates) in broth media for 12 h with subsequent survival recovery on agar plates. Additionally, agar plates were precoated with ILP100 at clinical doses (3 vs. 24 h, 1 \u00d7 107 CFU/cm2) followed by co-culture with pathogens inoculated in soft agar (1 \u00d7 104 CFU/cm2). To compare ILP100 with relevant antibiotics, MDR-inoculated soft agar was applied to plates with standardized ILP100 drops and antibiotic-loaded discs, followed by 18-20 h aerobic incubation at 37 \u00b0C.\n\nDose-dependent growth inhibition of all pathogens was demonstrated, as 1000:1 and 100:1 (ILP100/isolate) inhibited pathogenic growth up to log 6.4 and log 4.3 CFU/ml, respectively. Potent antimicrobial effects were demonstrated after precoating with ILP100, as pathogen recovery was only demonstrated after 3 h of precoating, only for 10/18 isolates and then only partially. Benchmarking to relevant antibiotic discs resulted in large cleared zones surrounding the ILP100 spots but not the antibiotic discs, demonstrating potent bacterial killing by ILP100-secreted factors. Interestingly, the MDR pathogens were significantly more sensitive to the ILP100 released factors than the non-MDR isolates.\n\nILP100 effectively eliminates MDR wound pathogens, which reveals a promising strategy for the development of new classes of urgently needed antimicrobials.", "doi": "10.1007/s40121-025-01137-y", "pmid": "40164924", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12084475"}, {"db": "pii", "key": "10.1007/s40121-025-01137-y"}], "notes": [], "created": "2026-08-20T06:40:52.265Z", "modified": "2026-08-20T06:40:52.357Z"}