{"entity": "publication", "iuid": "7130ae933f1840bfa2f2d8ff83659ff0", "timestamp": "2026-09-07T11:04:39.064Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7130ae933f1840bfa2f2d8ff83659ff0.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7130ae933f1840bfa2f2d8ff83659ff0"}}, "title": "A fluorescence nanoscopy marker for corticotropin-releasing hormone type 1 receptor: computer design, synthesis, signaling effects, super-resolved fluorescence imaging, and in situ affinity constant in cells.", "authors": [{"family": "Szalai", "given": "Alan M", "initials": "AM"}, {"family": "Armando", "given": "Natalia G", "initials": "NG"}, {"family": "Barabas", "given": "Federico M", "initials": "FM"}, {"family": "Stefani", "given": "Fernando D", "initials": "FD"}, {"family": "Giordano", "given": "Luciana", "initials": "L"}, {"family": "Bari", "given": "Sara E", "initials": "SE"}, {"family": "Cavasotto", "given": "Claudio N", "initials": "CN"}, {"family": "Silberstein", "given": "Susana", "initials": "S"}, {"family": "Aramend\u00eda", "given": "Pedro F", "initials": "PF"}], "type": "journal article", "published": "2018-11-28", "journal": {"title": "Phys Chem Chem Phys", "issn": "1463-9084", "volume": "20", "issue": "46", "pages": "29212-29220", "issn-l": "1463-9076"}, "abstract": "Class B G protein-coupled receptors (GPCRs) are involved in a variety of human pathophysiological states. These groups of membrane receptors are less studied than class A GPCRs due to the lack of structural information, delayed small molecule drug discovery, and scarce fluorescence detection tools available. The class B corticotropin-releasing hormone type 1 receptor (CRHR1) is a key player in the stress response whose dysregulation is critically involved in stress-related disorders: psychiatric conditions (i.e. depression, anxiety, and addictions), neuroendocrinological alterations, and neurodegenerative diseases. Here, we present a strategy to label GPCRs with a small fluorescent antagonist that permits the observation of the receptor in live cells through stochastic optical reconstruction microscopy (STORM) with 23 nm resolution. The marker, an aza-BODIPY derivative, was designed based on computational docking studies, then synthesized, and finally tested in biological cells. Experiments on hippocampal neurons demonstrate antagonist effects in similar concentrations as the well-established antagonist CP-376395. A quantitative analysis of two color STORM images enabled the determination of the binding affinity of the new marker in the cellular environment.", "doi": "10.1039/c8cp06196c", "pmid": "30427333", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-21T11:57:41.216Z", "modified": "2026-08-21T11:57:41.229Z"}