{"entity": "publication", "iuid": "6a9be1e5d3024b00853844855e4fbead", "timestamp": "2026-07-22T17:46:28.888Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/6a9be1e5d3024b00853844855e4fbead.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/6a9be1e5d3024b00853844855e4fbead"}}, "title": "Toward next generation plasma profiling via heat-induced epitope retrieval and array-based assays.", "authors": [{"family": "Schwenk", "given": "Jochen M", "initials": "JM"}, {"family": "Igel", "given": "Ulrika", "initials": "U"}, {"family": "Neiman", "given": "Maja", "initials": "M"}, {"family": "Langen", "given": "Hanno", "initials": "H"}, {"family": "Becker", "given": "Charlotte", "initials": "C"}, {"family": "Bjartell", "given": "Anders", "initials": "A"}, {"family": "Ponten", "given": "Fredrik", "initials": "F"}, {"family": "Wiklund", "given": "Fredrik", "initials": "F"}, {"family": "Gr\u00f6nberg", "given": "Henrik", "initials": "H"}, {"family": "Nilsson", "given": "Peter", "initials": "P"}, {"family": "Uhlen", "given": "Mathias", "initials": "M"}], "type": "evaluation studies", "published": "2010-11-00", "journal": {"title": "Mol. Cell Proteomics", "issn": "1535-9484", "volume": "9", "issue": "11", "pages": "2497-2507", "issn-l": "1535-9476"}, "abstract": "There is a need for high throughput methods for screening patient samples in the quest for potential biomarkers for diagnostics and patient care. Here, we used a combination of undirected target selection, antibody suspension bead arrays, and heat-induced epitope retrieval to allow for protein profiling of human plasma in a novel and systematic manner. Several antibodies were found to reveal altered protein profiles upon epitope retrieval at elevated temperatures with limits of detection improving into lower ng/ml ranges. In a study based on prostate cancer patients, several proteins with differential profiles were discovered and subsequently validated in an independent cohort. For one of the potential biomarkers, the human carnosine dipeptidase 1 protein (CNDP1), the differences were determined to be related to the glycosylation status of the targeted protein. The study shows a path of pursuit for large scale screening of biobank repositories in a flexible and proteome-wide fashion by utilizing heat-induced epitope retrieval and using an antibody suspension bead array format.", "doi": "10.1074/mcp.M110.001560", "pmid": "20682762", "labels": {"Affiliated researcher": null}, "xrefs": [{"db": "pii", "key": "M110.001560"}, {"db": "pmc", "key": "PMC2984230"}], "notes": [], "created": "2018-12-05T09:04:20.845Z", "modified": "2018-12-05T09:04:20.866Z"}