{"entity": "publication", "iuid": "62460327b2854760a878445d805ffb3b", "timestamp": "2026-10-14T21:43:57.853Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/62460327b2854760a878445d805ffb3b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/62460327b2854760a878445d805ffb3b"}}, "title": "Adenoviral detection by recombinase polymerase amplification and vertical flow paper microarray.", "authors": [{"family": "Nybond", "given": "Susanna", "initials": "S"}, {"family": "R\u00e9u", "given": "Pedro", "initials": "P"}, {"family": "Rhedin", "given": "Samuel", "initials": "S"}, {"family": "Svedberg", "given": "Gustav", "initials": "G"}, {"family": "Alfv\u00e9n", "given": "Tobias", "initials": "T"}, {"family": "Gantelius", "given": "Jesper", "initials": "J"}, {"family": "Svahn", "given": "Helene Andersson", "initials": "HA"}], "type": "journal article", "published": "2019-02-00", "journal": {"title": "Anal Bioanal Chem", "issn": "1618-2650", "volume": "411", "issue": "4", "pages": "813-822", "issn-l": "1618-2642"}, "abstract": "Respiratory viral infections often mimic the symptoms of infections caused by bacteria; however, restricted and targeted administration of antibiotics is needed to combat growing antimicrobial resistance. This is particularly relevant in low-income settings. In this work, we describe the use of isothermal amplification of viral DNA at 37 \u00b0C coupled to a paper-based vertical flow microarray (VFM) setup that utilizes a colorimetric detection of amplicons using functionalized gold nanoparticles. Two oligonucleotide probes, one in-house designed and one known adenoviral probe were tested and validated for microarray detection down to 50 nM using a synthetic target template. Furthermore, primers were shown to function in a recombinase polymerase amplification reaction using both synthetic template and viral DNA. As a proof-of-concept, we demonstrate adenoviral detection with four different adenoviral species associated with respiratory infections using the paper-based VFM format. The presented assay was validated with selected adenoviral species using the in-house probe, enabling detection at 1 ng of starting material with intra- and inter-assay %CV of \u2264 9% and \u2264 13%. Finally, we validate our overall method using clinical samples. Based on the results, the combination of recombinase polymerase amplification, paper microarray analysis, and nanoparticle-based colorimetric detection could thus be a useful strategy towards rapid and affordable multiplexed viral diagnostics.", "doi": "10.1007/s00216-018-1503-y", "pmid": "30498984", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6338793"}, {"db": "pii", "key": "10.1007/s00216-018-1503-y"}], "notes": [], "created": "2026-10-01T08:27:26.632Z", "modified": "2026-10-01T08:27:26.647Z"}