{"entity": "publication", "iuid": "623871df9ac243d8a817b9d8f9b50fdc", "timestamp": "2026-08-20T21:30:45.945Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/623871df9ac243d8a817b9d8f9b50fdc.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/623871df9ac243d8a817b9d8f9b50fdc"}}, "title": "Extracellular Vesicle Capture by AnTibody of CHoice and Enzymatic Release (EV-CATCHER): A customizable purification assay designed for small-RNA biomarker identification and evaluation of circulating small-EVs.", "authors": [{"family": "Mitchell", "given": "Megan I", "initials": "MI", "orcid": "0000-0002-1202-155X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/87c65c9726654b22a899be623395908c.json"}}, {"family": "Ben-Dov", "given": "Iddo Z", "initials": "IZ"}, {"family": "Liu", "given": "Christina", "initials": "C"}, {"family": "Ye", "given": "Kenny", "initials": "K"}, {"family": "Chow", "given": "Kar", "initials": "K"}, {"family": "Kramer", "given": "Yael", "initials": "Y"}, {"family": "Gangadharan", "given": "Anju", "initials": "A"}, {"family": "Park", "given": "Steven", "initials": "S"}, {"family": "Fitzgerald", "given": "Sean", "initials": "S"}, {"family": "Ramnauth", "given": "Andrew", "initials": "A"}, {"family": "Perlin", "given": "David S", "initials": "DS"}, {"family": "Donato", "given": "Michele", "initials": "M"}, {"family": "Bhoy", "given": "Emily", "initials": "E"}, {"family": "Manouchehri Doulabi", "given": "Ehsan", "initials": "E"}, {"family": "Poulos", "given": "Michael", "initials": "M"}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M"}, {"family": "Loudig", "given": "Olivier", "initials": "O"}], "type": "journal article", "published": "2021-06-00", "journal": {"title": "J Extracell Vesicles", "issn": "2001-3078", "volume": "10", "issue": "8", "pages": "e12110", "issn-l": null}, "abstract": "Circulating nucleic acids, encapsulated within small extracellular vesicles (EVs), provide a remote cellular snapshot of biomarkers derived from diseased tissues, however selective isolation is critical. Current laboratory-based purification techniques rely on the physical properties of small-EVs rather than their inherited cellular fingerprints. We established a highly-selective purification assay, termed EV-CATCHER, initially designed for high-throughput analysis of low-abundance small-RNA cargos by next-generation sequencing. We demonstrated its selectivity by specifically isolating and sequencing small-RNAs from mouse small-EVs spiked into human plasma. Western blotting, nanoparticle tracking, and transmission electron microscopy were used to validate and quantify the capture and release of intact small-EVs. As proof-of-principle for sensitive detection of circulating miRNAs, we compared small-RNA sequencing data from a subset of small-EVs serum-purified with EV-CATCHER to data from whole serum, using samples from a small cohort of recently hospitalized Covid-19 patients. We identified and validated, only in small-EVs, hsa-miR-146a and hsa-miR-126-3p to be significantly downregulated with disease severity. Separately, using convalescent sera from recovered Covid-19 patients with high anti-spike IgG titers, we confirmed the neutralizing properties, against SARS-CoV-2 in vitro, of a subset of small-EVs serum-purified by EV-CATCHER, as initially observed with ultracentrifuged small-EVs. Altogether our data highlight the sensitivity and versatility of EV-CATCHER.", "doi": "10.1002/jev2.12110", "pmid": "34122779", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8173589"}, {"db": "pii", "key": "JEV212110"}], "notes": [], "created": "2026-08-20T06:34:05.937Z", "modified": "2026-08-20T06:34:06.049Z"}