{"entity": "publication", "iuid": "617edd69409e4be8b0615205dac1ab64", "timestamp": "2026-08-20T20:49:51.052Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/617edd69409e4be8b0615205dac1ab64.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/617edd69409e4be8b0615205dac1ab64"}}, "title": "A Retro-biosynthesis-Based Route to Generate Pinene-Derived Polyesters.", "authors": [{"family": "Stamm", "given": "Arne", "initials": "A"}, {"family": "Biundo", "given": "Antonino", "initials": "A"}, {"family": "Schmidt", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Br\u00fccher", "given": "J\u00f6rg", "initials": "J"}, {"family": "Lundmark", "given": "Stefan", "initials": "S"}, {"family": "Ols\u00e9n", "given": "Peter", "initials": "P"}, {"family": "Fogelstr\u00f6m", "given": "Linda", "initials": "L"}, {"family": "Malmstr\u00f6m", "given": "Eva", "initials": "E"}, {"family": "Bornscheuer", "given": "Uwe T", "initials": "UT"}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal article", "published": "2019-07-01", "journal": {"title": "Chembiochem", "issn": "1439-7633", "volume": "20", "issue": "13", "pages": "1664-1671", "issn-l": "1439-4227"}, "abstract": "Significantly increased production of biobased polymers is a prerequisite to replace petroleum-based materials towards reaching a circular bioeconomy. However, many renewable building blocks from wood and other plant material are not directly amenable for polymerization, due to their inert backbones and/or lack of functional group compatibility with the desired polymerization type. Based on a retro-biosynthetic analysis of polyesters, a chemoenzymatic route from (-)-\u03b1-pinene towards a verbanone-based lactone, which is further used in ring-opening polymerization, is presented. Generated pinene-derived polyesters showed elevated degradation and glass transition temperatures, compared with poly(\u03f5-decalactone), which lacks a ring structure in its backbone. Semirational enzyme engineering of the cyclohexanone monooxygenase from Acinetobacter calcoaceticus enabled the biosynthesis of the key lactone intermediate for the targeted polyester. As a proof of principle, one enzyme variant identified from screening in a microtiter plate was used in biocatalytic upscaling, which afforded the bicyclic lactone in 39 % conversion in shake flask scale reactions.", "doi": "10.1002/cbic.201900046", "pmid": "30793830", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6618282"}], "notes": [], "created": "2026-08-20T06:30:49.146Z", "modified": "2026-08-20T06:30:49.216Z"}