{"entity": "publication", "iuid": "6052e21715bd488694b5fbb77617fc26", "timestamp": "2026-08-22T06:56:51.047Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/6052e21715bd488694b5fbb77617fc26.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/6052e21715bd488694b5fbb77617fc26"}}, "title": "Pulmonary and blood dendritic cells from sarcoidosis patients more potently induce IFN\u03b3-producing Th1 cells compared with monocytes.", "authors": [{"family": "Lepzien", "given": "Rico", "initials": "R"}, {"family": "Nie", "given": "Mu", "initials": "M"}, {"family": "Czarnewski", "given": "Paulo", "initials": "P"}, {"family": "Liu", "given": "Sang", "initials": "S"}, {"family": "Yu", "given": "Meng", "initials": "M"}, {"family": "Ravindran", "given": "Avinash", "initials": "A"}, {"family": "Kullberg", "given": "Susanna", "initials": "S"}, {"family": "Eklund", "given": "Anders", "initials": "A"}, {"family": "Grunewald", "given": "Johan", "initials": "J"}, {"family": "Smed-S\u00f6rensen", "given": "Anna", "initials": "A"}], "type": "journal article", "published": "2022-04-00", "journal": {"title": "J Leukoc Biol", "issn": "1938-3673", "volume": "111", "issue": "4", "pages": "857-866", "issn-l": "0741-5400"}, "abstract": "Sarcoidosis is a systemic inflammatory disease mainly affecting the lungs. The hallmark of sarcoidosis are granulomas that are surrounded by activated T cells, likely targeting the disease-inducing antigen. IFN\u03b3-producing Th1 and Th17.1 T cells are elevated in sarcoidosis and associate with disease progression. Monocytes and dendritic cells (DCs) are antigen-presenting cells (APCs) and required for T cell activation. Several subsets of monocytes and DCs with different functions were identified in sarcoidosis. However, to what extent different monocyte and DC subsets can support activation and skewing of T cells in sarcoidosis is still unclear. In this study, we performed a transcriptional and functional side-by-side comparison of sorted monocytes and DCs from matched blood and bronchoalveolar lavage (BAL) fluid of sarcoidosis patients. Transcriptomic analysis of all subsets showed upregulation of genes related to T cell activation and antigen presentation in DCs compared with monocytes. Allogeneic T cell proliferation was higher after coculture with monocytes and DCs from blood compared with BAL and DCs induced more T cell proliferation compared with monocytes. After coculture, proliferating T cells showed high expression of the transcription factor Tbet and IFN\u03b3 production. We also identified Tbet and ROR\u03b3t coexpressing T cells that mainly produced IFN\u03b3. Our data show that DCs rather than monocytes from sarcoidosis patients have the ability to activate and polarize T cells towards Th1 and Th17.1 cells. This study provides a useful in vitro tool to better understand the contribution of monocytes and DCs to T cell activation and immunopathology in sarcoidosis.", "doi": "10.1002/JLB.5A0321-162R", "pmid": "34431542", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-21T10:59:06.616Z", "modified": "2026-08-21T10:59:06.640Z"}