{"entity": "publication", "iuid": "5df0f2a0723d4f13926d55490984aa52", "timestamp": "2026-08-20T21:02:17.730Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5df0f2a0723d4f13926d55490984aa52.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5df0f2a0723d4f13926d55490984aa52"}}, "title": "Comparative evaluation of tumor targeting using the anti-HER2 ADAPT scaffold protein labeled at the C-terminus with indium-111 or technetium-99m.", "authors": [{"family": "Garousi", "given": "Javad", "initials": "J"}, {"family": "Lindbo", "given": "Sarah", "initials": "S"}, {"family": "Mitran", "given": "Bogdan", "initials": "B"}, {"family": "Buijs", "given": "Jos", "initials": "J", "orcid": "0000-0003-3955-1320", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b8ab0f168ed348baa89d0578fc9c78a1.json"}}, {"family": "Vorobyeva", "given": "Anzhelika", "initials": "A"}, {"family": "Orlova", "given": "Anna", "initials": "A"}, {"family": "Tolmachev", "given": "Vladimir", "initials": "V"}, {"family": "Hober", "given": "Sophia", "initials": "S"}], "type": "comparative study", "published": "2017-11-07", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "7", "issue": "1", "pages": "14780", "issn-l": "2045-2322"}, "abstract": "ABD-Derived Affinity Proteins (ADAPTs) is a novel class of engineered scaffold proteins derived from an albumin-binding domain of protein G. The use of ADAPT6 derivatives as targeting moiety have provided excellent preclinical radionuclide imaging of human epidermal growth factor 2 (HER2) tumor xenografts. Previous studies have demonstrated that selection of nuclide and chelator for its conjugation has an appreciable effect on imaging properties of scaffold proteins. In this study we performed a comparative evaluation of the anti-HER2 ADAPT having an aspartate-glutamate-alanine-valine-aspartate-alanine-asparagine-serine (DEAVDANS) N-terminal sequence and labeled at C-terminus with 99mTc using a cysteine-containing peptide based chelator, glycine-serine-serine-cysteine (GSSC), and a similar variant labeled with 111In using a maleimido derivative of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) chelator. Both 99mTc-DEAVDANS-ADAPT6-GSSC and 111In-DEAVDANS-ADAPT6-GSSC-DOTA accumulated specifically in HER2-expressing SKOV3 xenografts. The tumor uptake of both variants did not differ significantly and average values were in the range of 19-21%ID/g. However, there was an appreciable variation in uptake of conjugates in normal tissues that resulted in a notable difference in the tumor-to-organ ratios. The 111In-DOTA label provided 2-6 fold higher tumor-to-organ ratios than 99mTc-GSSC and is therefore the preferable label for ADAPTs.", "doi": "10.1038/s41598-017-15366-w", "pmid": "29116215", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC5676751"}, {"db": "pii", "key": "10.1038/s41598-017-15366-w"}], "notes": [], "created": "2026-08-20T09:04:17.421Z", "modified": "2026-08-20T09:04:17.442Z"}