{"entity": "publication", "iuid": "5ac786f7abdb4c1389d9cd05239148b3", "timestamp": "2026-08-25T09:30:46.698Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5ac786f7abdb4c1389d9cd05239148b3.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5ac786f7abdb4c1389d9cd05239148b3"}}, "title": "An engineered thermal-shift screen reveals specific lipid preferences of eukaryotic and prokaryotic membrane proteins.", "authors": [{"family": "Nji", "given": "Emmanuel", "initials": "E", "orcid": "0000-0001-6991-1046", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/daa539e0d91849c6801b6ad9c4c351c5.json"}}, {"family": "Chatzikyriakidou", "given": "Yurie", "initials": "Y"}, {"family": "Landreh", "given": "Michael", "initials": "M"}, {"family": "Drew", "given": "David", "initials": "D"}], "type": "journal article", "published": "2018-10-12", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "9", "issue": "1", "pages": "4253", "issn-l": "2041-1723"}, "abstract": "Membrane bilayers are made up of a myriad of different lipids that regulate the functional activity, stability, and oligomerization of many membrane proteins. Despite their importance, screening the structural and functional impact of lipid-protein interactions to identify specific lipid requirements remains a major challenge. Here, we use the FSEC-TS assay to show cardiolipin-dependent stabilization of the dimeric sodium/proton antiporter NhaA, demonstrating its ability to detect specific protein-lipid interactions. Based on the principle of FSEC-TS, we then engineer a simple thermal-shift assay (GFP-TS), which facilitates the high-throughput screening of lipid- and ligand- interactions with membrane proteins. By comparing the thermostability of medically relevant eukaryotic membrane proteins and a selection of bacterial counterparts, we reveal that eukaryotic proteins appear to have evolved to be more dependent to the presence of specific lipids.", "doi": "10.1038/s41467-018-06702-3", "pmid": "30315156", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6185904"}, {"db": "pii", "key": "10.1038/s41467-018-06702-3"}], "notes": [], "created": "2026-08-20T08:50:18.480Z", "modified": "2026-08-20T08:50:18.586Z"}