{"entity": "publication", "iuid": "57c9915b90d24c799504acb9feb42315", "timestamp": "2026-08-20T21:05:31.915Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/57c9915b90d24c799504acb9feb42315.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/57c9915b90d24c799504acb9feb42315"}}, "title": "Cellular responses to silencing of PDIA3 (protein disulphide-isomerase A3): Effects on proliferation, migration, and genes in control of active vitamin D.", "authors": [{"family": "Kermpatsou", "given": "Despoina", "initials": "D"}, {"family": "Olsson", "given": "Frida", "initials": "F"}, {"family": "W\u00e5hl\u00e9n", "given": "Erik", "initials": "E"}, {"family": "S\u00f6derberg", "given": "Ola", "initials": "O"}, {"family": "Lennartsson", "given": "Johan", "initials": "J"}, {"family": "Norlin", "given": "Maria", "initials": "M"}], "type": "journal article", "published": "2024-06-00", "journal": {"title": "J. Steroid Biochem. Mol. Biol.", "issn": "1879-1220", "volume": "240", "pages": "106497", "issn-l": "0960-0760"}, "abstract": "The active form of vitamin D, 1,25-dihydroxyvitamin D3, is known to act via VDR (vitamin D receptor), affecting several physiological processes. In addition, PDIA3 (protein disulphide-isomerase A3) has been associated with some of the functions of 1,25-dihydroxyvitamin D3. In the present study we used siRNA-mediated silencing of PDIA3 in osteosarcoma and prostate carcinoma cell lines to examine the role(s) of PDIA3 for 1,25-dihydroxyvitamin D3-dependent responses. PDIA3 silencing affected VDR target genes and significantly altered the 1,25-dihydroxyvitamin D3-dependent induction of CYP24A1, essential for elimination of excess 1,25-dihydroxyvitamin D3. Also, PDIA3 silencing significantly altered migration and proliferation in prostate PC3 cells, independently of 1,25-dihydroxyvitamin D3. 1,25-Dihydroxyvitamin D3 increased thermostability of PDIA3 in cellular thermal shift assay, supporting functional interaction between PDIA3 and 1,25-dihydroxyvitamin D3-dependent pathways. In summary, our data link PDIA3 to 1,25-dihydroxyvitamin D3-mediated signalling, underline and extend its role in proliferation and reveal a novel function in maintenance of 1,25-dihydroxyvitamin D3 levels.", "doi": "10.1016/j.jsbmb.2024.106497", "pmid": "38460707", "labels": [], "xrefs": [{"db": "pii", "key": "S0960-0760(24)00045-1"}], "notes": [], "created": "2026-08-20T08:00:09.260Z", "modified": "2026-08-20T08:00:09.307Z"}