{"entity": "publication", "iuid": "54612b531cc6492e8e1a4dabe08fe65d", "timestamp": "2026-08-20T21:01:42.396Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/54612b531cc6492e8e1a4dabe08fe65d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/54612b531cc6492e8e1a4dabe08fe65d"}}, "title": "Indirect 3D Bioprinting of a Robust Trilobular Hepatic Construct with Decellularized Liver Matrix Hydrogel.", "authors": [{"family": "Khati", "given": "Vamakshi", "initials": "V"}, {"family": "Turkki", "given": "Johannes Artturi", "initials": "JA"}, {"family": "Ramachandraiah", "given": "Harisha", "initials": "H"}, {"family": "Pati", "given": "Falguni", "initials": "F", "orcid": "0000-0002-3588-1800", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a980f4446de44c9ca89931a1c774f1e6.json"}}, {"family": "Gaudenzi", "given": "Giulia", "initials": "G"}, {"family": "Russom", "given": "Aman", "initials": "A"}], "type": "journal article", "published": "2022-10-22", "journal": {"title": "Bioengineering (Basel)", "issn": "2306-5354", "volume": "9", "issue": "11", "issn-l": null}, "abstract": "The liver exhibits complex geometrical morphologies of hepatic cells arranged in a hexagonal lobule with an extracellular matrix (ECM) organized in a specific pattern on a multi-scale level. Previous studies have utilized 3D bioprinting and microfluidic perfusion systems with various biomaterials to develop lobule-like constructs. However, they all lack anatomical relevance with weak control over the size and shape of the fabricated structures. Moreover, most biomaterials lack liver-specific ECM components partially or entirely, which might limit their biomimetic mechanical properties and biological functions. Here, we report 3D bioprinting of a sacrificial PVA framework to impart its trilobular hepatic structure to the decellularized liver extracellular matrix (dLM) hydrogel with polyethylene glycol-based crosslinker and tyrosinase to fabricate a robust multi-scale 3D liver construct. The 3D trilobular construct exhibits higher crosslinking, viscosity (182.7 \u00b1 1.6 Pa\u00b7s), and storage modulus (2554 \u00b1 82.1 Pa) than non-crosslinked dLM. The co-culture of HepG2 liver cells and NIH 3T3 fibroblast cells exhibited the influence of fibroblasts on liver-specific activity over time (7 days) to show higher viability (90-91.5%), albumin secretion, and increasing activity of four liver-specific genes as compared to the HepG2 monoculture. This technique offers high lumen patency for the perfusion of media to fabricate a densely populated scaled-up liver model, which can also be extended to other tissue types with different biomaterials and multiple cells to support the creation of a large functional complex tissue.", "doi": "10.3390/bioengineering9110603", "pmid": "36354514", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9687301"}, {"db": "pii", "key": "bioengineering9110603"}], "notes": [], "created": "2026-08-20T13:38:30.250Z", "modified": "2026-08-20T13:38:30.359Z"}