{"entity": "publication", "iuid": "526a913e3ddb4d6ca19e18ff12183adf", "timestamp": "2026-08-20T21:26:28.440Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/526a913e3ddb4d6ca19e18ff12183adf.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/526a913e3ddb4d6ca19e18ff12183adf"}}, "title": "Generation of Anterior Segment of the Eye Cells from hiPSCs in Microfluidic Platforms.", "authors": [{"family": "Ko\u00e7ak", "given": "Gamze", "initials": "G", "orcid": "0000-0003-1164-367X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/70f4674b253d4eea953180e493478835.json"}}, {"family": "Uyulgan", "given": "Sude", "initials": "S", "orcid": "0000-0003-2188-061X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9e5cc0c2e7a34e6b90c5bf0e38a230f6.json"}}, {"family": "Polatl\u0131", "given": "Elifsu", "initials": "E", "orcid": "0000-0002-8960-4534", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/da4b34be608e4a16b1b9c733e833f5d4.json"}}, {"family": "Sar\u0131", "given": "Vedat", "initials": "V", "orcid": "0000-0002-3891-5869", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8be34175d8c5454794f22cb78a715426.json"}}, {"family": "Kahveci", "given": "Burak", "initials": "B", "orcid": "0000-0001-7041-0986", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/32564c59d64c4f6587a7902f58b9c0ba.json"}}, {"family": "Bursali", "given": "Ahmet", "initials": "A"}, {"family": "Binokay", "given": "Leman", "initials": "L", "orcid": "0000-0001-6162-3947", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3abf879d5525415a9e92938a8fb4569e.json"}}, {"family": "Re\u00e7ber", "given": "Tuba", "initials": "T", "orcid": "0000-0001-8257-7628", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bff4cbdba9fb47ffba373b171e66bdd0.json"}}, {"family": "Nemutlu", "given": "Emirhan", "initials": "E", "orcid": "0000-0002-7337-6215", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3779431f902b4e2fa9f04f13785195f9.json"}}, {"family": "Mardino\u011flu", "given": "Adil", "initials": "A"}, {"family": "Karak\u00fclah", "given": "G\u00f6khan", "initials": "G", "orcid": "0000-0001-6706-1375", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9f458878fc8c4980ab867aa3dce34228.json"}}, {"family": "Utine", "given": "Canan Asl\u0131", "initials": "CA", "orcid": "0000-0002-4131-2532", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ce631edc5eea476589c566367fc3c11e.json"}}, {"family": "G\u00fcven", "given": "Sinan", "initials": "S", "orcid": "0000-0001-5212-5516", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8248c62bca5243a18f31a974c2374920.json"}}], "type": "journal article", "published": "2024-05-00", "journal": {"title": "Adv Biol (Weinh)", "issn": "2701-0198", "volume": "8", "issue": "5", "pages": "e2400018", "issn-l": null}, "abstract": "Ophthalmic diseases affect many people, causing partial or total loss of vision and a reduced quality of life. The anterior segment of the eye accounts for nearly half of all visual impairment that can lead to blindness. Therefore, there is a growing demand for ocular research and regenerative medicine that specifically targets the anterior segment to improve vision quality. This study aims to generate a microfluidic platform for investigating the formation of the anterior segment of the eye derived from human induced pluripotent stem cells (hiPSC) under various spatial-mechanoresponsive conditions. Microfluidic platforms are developed to examine the effects of dynamic conditions on the generation of hiPSCs-derived ocular organoids. The differentiation protocol is validated, and mechanoresponsive genes are identified through transcriptomic analysis. Several culture strategies is implemented for the anterior segment of eye cells in a microfluidic chip. hiPSC-derived cells showed anterior eye cell characteristics in mRNA and protein expression levels under dynamic culture conditions. The expression levels of yes-associated protein and transcriptional coactivator PDZ binding motif (YAP/TAZ) and PIEZO1, varied depending on the differentiation and growth conditions of the cells, as well as the metabolomic profiles under dynamic culture conditions.", "doi": "10.1002/adbi.202400018", "pmid": "38640945", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:27:23.467Z", "modified": "2026-08-20T06:27:23.995Z"}