{"entity": "publication", "iuid": "49f2871269ad4feebc70d96479153e2d", "timestamp": "2026-08-23T09:25:45.580Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/49f2871269ad4feebc70d96479153e2d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/49f2871269ad4feebc70d96479153e2d"}}, "title": "Structural basis of LRPPRC-SLIRP-dependent translation by the mitoribosome.", "authors": [{"family": "Singh", "given": "Vivek", "initials": "V"}, {"family": "Moran", "given": "J Conor", "initials": "JC"}, {"family": "Itoh", "given": "Yuzuru", "initials": "Y"}, {"family": "Soto", "given": "Iliana C", "initials": "IC"}, {"family": "Fontanesi", "given": "Flavia", "initials": "F"}, {"family": "Couvillion", "given": "Mary", "initials": "M"}, {"family": "Huynen", "given": "Martijn A", "initials": "MA"}, {"family": "Churchman", "given": "L Stirling", "initials": "LS"}, {"family": "Barrientos", "given": "Antoni", "initials": "A"}, {"family": "Amunts", "given": "Alexey", "initials": "A", "orcid": "0000-0002-5302-1740", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6c79bee23e114906bc847dfbd0975139.json"}}], "type": "journal article", "published": "2024-12-00", "journal": {"title": "Nat. Struct. Mol. Biol.", "issn": "1545-9985", "volume": "31", "issue": "12", "pages": "1838-1847", "issn-l": "1545-9985"}, "abstract": "In mammalian mitochondria, mRNAs are cotranscriptionally stabilized by the protein factor LRPPRC (leucine-rich pentatricopeptide repeat-containing protein). Here, we characterize LRPPRC as an mRNA delivery factor and report its cryo-electron microscopy structure in complex with SLIRP (SRA stem-loop-interacting RNA-binding protein), mRNA and the mitoribosome. The structure shows that LRPPRC associates with the mitoribosomal proteins mS39 and the N terminus of mS31 through recognition of the LRPPRC helical repeats. Together, the proteins form a corridor for handoff of the mRNA. The mRNA is directly bound to SLIRP, which also has a stabilizing function for LRPPRC. To delineate the effect of LRPPRC on individual mitochondrial transcripts, we used RNA sequencing, metabolic labeling and mitoribosome profiling, which showed a transcript-specific influence on mRNA translation efficiency, with cytochrome c oxidase subunit 1 and 2 translation being the most affected. Our data suggest that LRPPRC-SLIRP acts in recruitment of mitochondrial mRNAs to modulate their translation. Collectively, the data define LRPPRC-SLIRP as a regulator of the mitochondrial gene expression system.", "doi": "10.1038/s41594-024-01365-9", "pmid": "39134711", "labels": [], "xrefs": [{"db": "mid", "key": "NIHMS2030709"}, {"db": "pmc", "key": "PMC11637978"}, {"db": "pii", "key": "10.1038/s41594-024-01365-9"}], "notes": [], "created": "2026-08-21T11:52:31.451Z", "modified": "2026-08-21T11:52:31.475Z"}