{"entity": "publication", "iuid": "48e34864ee6f41d49b039437a7396a47", "timestamp": "2026-09-24T14:46:21.042Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/48e34864ee6f41d49b039437a7396a47.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/48e34864ee6f41d49b039437a7396a47"}}, "title": "Single-cell BCR and transcriptome analysis after influenza infection reveals spatiotemporal dynamics of antigen-specific B cells.", "authors": [{"family": "Mathew", "given": "Nimitha R", "initials": "NR"}, {"family": "Jayanthan", "given": "Jayalal K", "initials": "JK"}, {"family": "Smirnov", "given": "Ilya V", "initials": "IV"}, {"family": "Robinson", "given": "Jonathan L", "initials": "JL"}, {"family": "Axelsson", "given": "Hannes", "initials": "H"}, {"family": "Nakka", "given": "Sravya S", "initials": "SS"}, {"family": "Emmanouilidi", "given": "Aikaterini", "initials": "A"}, {"family": "Czarnewski", "given": "Paulo", "initials": "P"}, {"family": "Yewdell", "given": "William T", "initials": "WT"}, {"family": "Sch\u00f6n", "given": "Karin", "initials": "K"}, {"family": "Lebrero-Fern\u00e1ndez", "given": "Cristina", "initials": "C"}, {"family": "Bernasconi", "given": "Valentina", "initials": "V"}, {"family": "Rodin", "given": "William", "initials": "W"}, {"family": "Harandi", "given": "Ali M", "initials": "AM"}, {"family": "Lycke", "given": "Nils", "initials": "N"}, {"family": "Borcherding", "given": "Nicholas", "initials": "N"}, {"family": "Yewdell", "given": "Jonathan W", "initials": "JW"}, {"family": "Greiff", "given": "Victor", "initials": "V"}, {"family": "Bemark", "given": "Mats", "initials": "M"}, {"family": "Angeletti", "given": "Davide", "initials": "D"}], "type": "journal article", "published": "2021-06-22", "journal": {"title": "Cell Reports", "issn": "2211-1247", "volume": "35", "issue": "12", "pages": "109286", "issn-l": null}, "abstract": "B cell responses are critical for antiviral immunity. However, a comprehensive picture of antigen-specific B cell differentiation, clonal proliferation, and dynamics in different organs after infection is lacking. Here, by combining single-cell RNA and B cell receptor (BCR) sequencing of antigen-specific cells in lymph nodes, spleen, and lungs after influenza infection in mice, we identify several germinal center (GC) B cell subpopulations and organ-specific differences that persist over the course of the response. We discover transcriptional differences between memory cells in lungs and lymphoid organs and organ-restricted clonal expansion. Remarkably, we find significant clonal overlap between GC-derived memory and plasma cells. By combining BCR-mutational analyses with monoclonal antibody (mAb) expression and affinity measurements, we find that memory B cells are highly diverse and can be selected from both low- and high-affinity precursors. By linking antigen recognition with transcriptional programming, clonal proliferation, and differentiation, these finding provide important advances in our understanding of antiviral immunity.", "doi": "10.1016/j.celrep.2021.109286", "pmid": "34161770", "labels": [], "xrefs": [{"db": "mid", "key": "EMS146188"}, {"db": "pmc", "key": "PMC7612943"}, {"db": "pii", "key": "S2211-1247(21)00657-4"}], "notes": [], "created": "2026-09-23T07:34:17.306Z", "modified": "2026-09-23T07:34:17.332Z"}