{"entity": "publication", "iuid": "420dba57c6a245ccb45c48fa4865206d", "timestamp": "2026-08-23T11:11:23.560Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/420dba57c6a245ccb45c48fa4865206d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/420dba57c6a245ccb45c48fa4865206d"}}, "title": "Rapid and sensitive detection of SARS-CoV-2 infection using quantitative peptide enrichment LC-MS analysis.", "authors": [{"family": "Hober", "given": "Andreas", "initials": "A", "orcid": "0000-0001-8947-2562", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/06cbc187628340278395a00ab9dd6c89.json"}}, {"family": "Tran-Minh", "given": "Khue Hua", "initials": "KH"}, {"family": "Foley", "given": "Dominic", "initials": "D"}, {"family": "McDonald", "given": "Thomas", "initials": "T"}, {"family": "Vissers", "given": "Johannes Pc", "initials": "JP"}, {"family": "Pattison", "given": "Rebecca", "initials": "R"}, {"family": "Ferries", "given": "Samantha", "initials": "S"}, {"family": "Hermansson", "given": "Sigurd", "initials": "S"}, {"family": "Betner", "given": "Ingvar", "initials": "I"}, {"family": "Uhl\u00e9n", "given": "Mathias", "initials": "M"}, {"family": "Razavi", "given": "Morteza", "initials": "M"}, {"family": "Yip", "given": "Richard", "initials": "R"}, {"family": "Pope", "given": "Matthew E", "initials": "ME"}, {"family": "Pearson", "given": "Terry W", "initials": "TW"}, {"family": "Andersson", "given": "Leigh N", "initials": "LN"}, {"family": "Bartlett", "given": "Amy", "initials": "A"}, {"family": "Calton", "given": "Lisa", "initials": "L"}, {"family": "Alm", "given": "Jessica J", "initials": "JJ"}, {"family": "Engstrand", "given": "Lars", "initials": "L"}, {"family": "Edfors", "given": "Fredrik", "initials": "F", "orcid": "0000-0002-0017-7987", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9e213b04f8984c8eb00c68f5bc2f6a36.json"}}], "type": "journal article", "published": "2021-11-08", "journal": {"title": "Elife", "issn": "2050-084X", "volume": "10", "issn-l": "2050-084X"}, "abstract": "Reliable, robust, large-scale molecular testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for monitoring the ongoing coronavirus disease 2019 (COVID-19) pandemic. We have developed a scalable analytical approach to detect viral proteins based on peptide immuno-affinity enrichment combined with liquid chromatography-mass spectrometry (LC-MS). This is a multiplexed strategy, based on targeted proteomics analysis and read-out by LC-MS, capable of precisely quantifying and confirming the presence of SARS-CoV-2 in phosphate-buffered saline (PBS) swab media from combined throat/nasopharynx/saliva samples. The results reveal that the levels of SARS-CoV-2 measured by LC-MS correlate well with their correspondingreal-time polymerase chain reaction (RT-PCR) read-out (r = 0.79). The analytical workflow shows similar turnaround times as regular RT-PCR instrumentation with a quantitative read-out of viral proteins corresponding to cycle thresholds (Ct) equivalents ranging from 21 to 34. Using RT-PCR as a reference, we demonstrate that the LC-MS-based method has 100% negative percent agreement (estimated specificity) and 95% positive percent agreement (estimated sensitivity) when analyzing clinical samples collected from asymptomatic individuals with a Ct within the limit of detection of the mass spectrometer (Ct \u2264 30). These results suggest that a scalable analytical method based on LC-MS has a place in future pandemic preparedness centers to complement current virus detection technologies.", "doi": "10.7554/eLife.70843", "pmid": "34747696", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8626084"}, {"db": "pii", "key": "70843"}], "notes": [], "created": "2026-08-21T13:07:44.123Z", "modified": "2026-08-21T13:07:44.212Z"}