{"entity": "publication", "iuid": "41b3e4b22f6d437485061c4c2f23b1a6", "timestamp": "2026-10-01T11:59:47.191Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/41b3e4b22f6d437485061c4c2f23b1a6.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/41b3e4b22f6d437485061c4c2f23b1a6"}}, "title": "Network-based insights into miRNA regulation of \u03b2-cell insulin secretion in type 2 diabetes.", "authors": [{"family": "Cowan", "given": "Elaine", "initials": "E"}, {"family": "Karagiannopoulos", "given": "Alexandros", "initials": "A"}, {"family": "Pollastri", "given": "Alessio", "initials": "A"}, {"family": "Asai", "given": "Akira", "initials": "A"}, {"family": "Nagao", "given": "Mototsugu", "initials": "M"}, {"family": "Maziarz", "given": "Marlena", "initials": "M"}, {"family": "Esguerra", "given": "Jonathan L S", "initials": "JLS"}, {"family": "Eliasson", "given": "Lena", "initials": "L"}], "type": "journal article", "published": "2025-12-19", "journal": {"title": "iScience", "issn": "2589-0042", "volume": "28", "issue": "12", "pages": "114200", "issn-l": null}, "abstract": "Increasing evidence suggests that microRNAs (miRNAs) contribute to pancreatic \u03b2-cell compensation during type 2 diabetes (T2D) pathogenesis. To examine miRNA-mRNA interactions in human islets and their roles in \u03b2-cell insulin secretion and T2D, we performed small-RNA sequencing on pancreatic islets from nine individuals with T2D and 52 non-diabetic controls. We identified 70 differentially expressed miRNAs, with miRNAs upregulated in T2D enriched in a co-expression network associated with insulin secretion. Eight such upregulated miRNAs, including miR-101-3p and miR-9-5p associated with both first- and second-phase insulin secretion. Among them, miR-101-3p had the most mRNA targets, while highly abundant mRNA transcripts (e.g., INS) were regulated by few miRNAs. Overexpression of miR-101-3p in \u03b2-cells increased insulin release in vitro and reduced expression of CADM1, a target of miR-101-3p. In summary, we have comprehensively identified miRNA-mRNA alterations in human islets associated with T2D pathogenesis and propose that miR-101-3p plays an important role in \u03b2-cell insulin secretion.", "doi": "10.1016/j.isci.2025.114200", "pmid": "41438030", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12721147"}, {"db": "pii", "key": "S2589-0042(25)02461-7"}], "notes": [], "created": "2026-10-01T08:27:20.928Z", "modified": "2026-10-01T08:27:20.953Z"}