{"entity": "publication", "iuid": "3e77c6a2c9894186b41dab6dcafe846f", "timestamp": "2026-08-22T06:51:03.924Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3e77c6a2c9894186b41dab6dcafe846f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3e77c6a2c9894186b41dab6dcafe846f"}}, "title": "Prognostic role of tumour-infiltrating lymphocytes and macrophages in relation to MSI, CDX2 and BRAF status: a population-based study of metastatic colorectal cancer patients.", "authors": [{"family": "Aaseb\u00f8", "given": "Kristine", "initials": "K"}, {"family": "Bruun", "given": "Jarle", "initials": "J"}, {"family": "Bergsland", "given": "Christian H", "initials": "CH", "orcid": "0000-0001-7700-8156", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6f2d3144994844da9a47b7557f9f2398.json"}}, {"family": "Nunes", "given": "Lu\u00eds", "initials": "L", "orcid": "0000-0002-3391-1607", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7aa31ccf1801456bb57744978bd605fd.json"}}, {"family": "Eide", "given": "Geir Egil", "initials": "GE"}, {"family": "Pfeiffer", "given": "Per", "initials": "P"}, {"family": "Dahl", "given": "Olav", "initials": "O"}, {"family": "Glimelius", "given": "Bengt", "initials": "B"}, {"family": "Lothe", "given": "Ragnhild A", "initials": "RA"}, {"family": "Sorbye", "given": "Halfdan", "initials": "H"}], "type": "journal article", "published": "2022-01-00", "journal": {"title": "Br. J. Cancer", "issn": "1532-1827", "volume": "126", "issue": "1", "pages": "48-56", "issn-l": "0007-0920"}, "abstract": "Tumour-infiltrating CD3, CD8 lymphocytes and CD68 macrophages are associated with favourable prognosis in localised colorectal cancer, but the effect in metastatic colorectal cancer (mCRC) is not established.\n\nA Scandinavian population-based cohort of non-resectable mCRC patients was studied. Tissue microarrays (n = 460) were stained with CD3, CD8 and CD68 using fluorescence-based multiplex immunohistochemistry. Associations with clinicopathological variables, overall survival (OS) and progression-free survival were estimated.\n\nTwo-thirds of microsatellite instable (MSI) and one-fourth of microsatellite stable (MSS) tumours displayed the highest quartile density of CD8. For CD3 high vs low cases, median OS was 20 vs 16 months (HR: 0.76, 95% CI: 0.59, 0.76, p = 0.025) with 3-year OS of 27 vs 13%. For CD68 high vs low cases, median OS was 23 vs 15 months (HR: 0.69, 95% CI: 0.54, 0.88, p = 0.003) with 3-year OS of 28 vs 12%. MSI, BRAF mutation and CDX2 loss were negative prognostic markers independent of tumour immune infiltration.\n\nIn mCRC, high lymphocyte infiltration was found in proportions of MSI and MSS tumours-potential subgroups of immunotherapy response. Tumour-infiltrating CD3 lymphocytes and CD68 macrophages were associated with median and long-term survival. MSI was a significant negative prognostic marker despite high immunogenicity.", "doi": "10.1038/s41416-021-01586-5", "pmid": "34671130", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8727629"}, {"db": "pii", "key": "10.1038/s41416-021-01586-5"}], "notes": [], "created": "2026-08-21T11:47:28.254Z", "modified": "2026-08-21T11:47:28.365Z"}