{"entity": "publication", "iuid": "39c339e607ea44db8c122daffb194c9a", "timestamp": "2026-08-22T06:57:19.786Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/39c339e607ea44db8c122daffb194c9a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/39c339e607ea44db8c122daffb194c9a"}}, "title": "miR-378a regulates keratinocyte responsiveness to interleukin-17A in psoriasis.", "authors": [{"family": "Xia", "given": "Ping", "initials": "P"}, {"family": "Pasquali", "given": "Lorenzo", "initials": "L"}, {"family": "Gao", "given": "Chenying", "initials": "C"}, {"family": "Srivastava", "given": "Ankit", "initials": "A"}, {"family": "Khera", "given": "Nupur", "initials": "N"}, {"family": "Freisenhausen", "given": "Jan Cedric", "initials": "JC"}, {"family": "Luo", "given": "Longlong", "initials": "L"}, {"family": "Ros\u00e9n", "given": "Einar", "initials": "E"}, {"family": "van Lierop", "given": "Anke", "initials": "A"}, {"family": "Homey", "given": "Bernhard", "initials": "B"}, {"family": "Pivarcsi", "given": "Andor", "initials": "A", "orcid": "0000-0003-2196-1102", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ebdb5779649e48be9801a25c72eaaa59.json"}}, {"family": "Sonkoly", "given": "Enik\u00f6", "initials": "E", "orcid": "0000-0002-4909-5413", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/89dff5a338334b41a8f5ce94f6595af2.json"}}], "type": "journal article", "published": "2022-08-00", "journal": {"title": "Br. J. Dermatol.", "issn": "1365-2133", "volume": "187", "issue": "2", "pages": "211-222", "issn-l": "0007-0963"}, "abstract": "Psoriasis is an immune-mediated inflammatory skin disease, in which an interplay between infiltrating immune cells and keratinocytes sustains chronic skin inflammation. Interleukin (IL)-17A is a key inflammatory cytokine in psoriasis and its main cellular targets are keratinocytes.\n\nTo explore the role of miR-378a in psoriasis.\n\nKeratinocytes obtained from psoriatic skin and healthy epidermis were separated by magnetic sorting, and the expression of miR-378a was analysed by quantitative polymerase chain reaction. The regulation and function of miR-378a was studied using primary human keratinocytes. The expression of miR-378a was modulated by synthetic mimics, and nuclear factor kappa B (NF-\u03baB) activity and transcriptomic changes were studied. Synthetic miR-378a was delivered to mouse skin in conjunction with induction of psoriasiform skin inflammation by imiquimod.\n\nWe show that miR-378a is induced by IL-17A in keratinocytes through NF-\u03baB, C/EBP-\u03b2 and I\u03baB\u03b6 and that it is overexpressed in psoriatic epidermis. In cultured keratinocytes, ectopic expression of miR-378a resulted in the nuclear translocation of p65 and enhanced NF-\u03baB-driven promoter activity even in the absence of inflammatory stimuli. Moreover, miR-378a potentiated the effect of IL-17A on NF-\u03baB nuclear translocation and downstream activation of the NF-\u03baB pathway. Finally, injection of miR-378a into mouse skin augmented psoriasis-like skin inflammation with increased epidermal proliferation and induction of inflammatory mediators. Mechanistically, miR-378a acts as a suppressor of NFKBIA/I\u03baB\u03b6, an important negative regulator of the NF-\u03baB pathway in keratinocytes.\n\nCollectively, our findings identify miR-378a as an amplifier of IL-17A-induced NF-\u03baB signalling in keratinocytes and suggest that increased miR-378a levels contribute to the amplification of IL-17A-driven skin inflammation in psoriasis.", "doi": "10.1111/bjd.21232", "pmid": "35257359", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9545829"}], "notes": [], "created": "2026-08-21T12:17:34.469Z", "modified": "2026-08-21T12:17:34.556Z"}