{"entity": "publication", "iuid": "33463bd996d148f7abd7b70bcdca36c1", "timestamp": "2026-08-20T21:22:41.570Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/33463bd996d148f7abd7b70bcdca36c1.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/33463bd996d148f7abd7b70bcdca36c1"}}, "title": "A distinctive family of L,D-transpeptidases catalyzing L-Ala-mDAP crosslinks in Alpha- and Betaproteobacteria.", "authors": [{"family": "Espaillat", "given": "Akbar", "initials": "A"}, {"family": "Alvarez", "given": "Laura", "initials": "L", "orcid": "0000-0003-2429-7542", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/329d8b1460fe44789dbef53ea0493294.json"}}, {"family": "Torrens", "given": "Gabriel", "initials": "G", "orcid": "0000-0002-0450-1430", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2a84794f089441ea8700f06f5a59fc2f.json"}}, {"family": "Ter Beek", "given": "Josy", "initials": "J", "orcid": "0000-0003-4165-9277", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3d76493e80b4b57a951a0f94e6123ca.json"}}, {"family": "Miguel-Ruano", "given": "Vega", "initials": "V", "orcid": "0000-0002-2492-7164", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ee0be393f7b44f3aeb6c926d39dfac9.json"}}, {"family": "Irazoki", "given": "Oihane", "initials": "O"}, {"family": "Gago", "given": "Federico", "initials": "F", "orcid": "0000-0002-3071-4878", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2f4b917616c44f69acace2feeb97763d.json"}}, {"family": "Hermoso", "given": "Juan A", "initials": "JA"}, {"family": "Berntsson", "given": "Ronnie P-A", "initials": "RP", "orcid": "0000-0001-6848-322X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cb9399248b784100a8020261949fb910.json"}}, {"family": "Cava", "given": "Felipe", "initials": "F", "orcid": "0000-0001-5995-718X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bbcff12a06814d54afea533e125a1213.json"}}], "type": "journal article", "published": "2024-02-13", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "1343", "issn-l": "2041-1723"}, "abstract": "The bacterial cell-wall peptidoglycan is made of glycan strands crosslinked by short peptide stems. Crosslinks are catalyzed by DD-transpeptidases (4,3-crosslinks) and LD-transpeptidases (3,3-crosslinks). However, recent research on non-model species has revealed novel crosslink types, suggesting the existence of uncharacterized enzymes. Here, we identify an LD-transpeptidase, LDTGo, that generates 1,3-crosslinks in the acetic-acid bacterium Gluconobacter oxydans. LDTGo-like proteins are found in Alpha- and Betaproteobacteria lacking LD3,3-transpeptidases. In contrast with the strict specificity of typical LD- and DD-transpeptidases, LDTGo can use non-terminal amino acid moieties for crosslinking. A high-resolution crystal structure of LDTGo reveals unique features when compared to LD3,3-transpeptidases, including a proline-rich region that appears to limit substrate access, and a cavity accommodating both glycan chain and peptide stem from donor muropeptides. Finally, we show that DD-crosslink turnover is involved in supplying the necessary substrate for LD1,3-transpeptidation. This phenomenon underscores the interplay between distinct crosslinking mechanisms in maintaining cell wall integrity in G. oxydans.", "doi": "10.1038/s41467-024-45620-5", "pmid": "38351082", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10864386"}, {"db": "pii", "key": "10.1038/s41467-024-45620-5"}], "notes": [], "created": "2026-08-20T08:53:08.705Z", "modified": "2026-08-20T08:53:08.995Z"}