{"entity": "publication", "iuid": "2618c3835c26421a8facdf5eeb52b33a", "timestamp": "2026-10-01T11:58:30.128Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/2618c3835c26421a8facdf5eeb52b33a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/2618c3835c26421a8facdf5eeb52b33a"}}, "title": "Anchored screening identifies transcription factor blueprints underlying dendritic cell diversity and subset-specific anti-tumor immunity.", "authors": [{"family": "Henriques-Oliveira", "given": "Lu\u00eds", "initials": "L"}, {"family": "Altman", "given": "Abigail R", "initials": "AR"}, {"family": "Kurochkin", "given": "Ilia", "initials": "I"}, {"family": "Ascic", "given": "Ervin", "initials": "E"}, {"family": "Halitzki", "given": "Evelyn", "initials": "E"}, {"family": "Matei", "given": "Andreea-Medeea", "initials": "AM"}, {"family": "P\u00e9rtiga-Cabral", "given": "Diogo", "initials": "D"}, {"family": "Ulmert", "given": "Isabel", "initials": "I"}, {"family": "Holst", "given": "Signe", "initials": "S"}, {"family": "Nair", "given": "Malavika Sreekumar", "initials": "MS"}, {"family": "Cunha", "given": "Pedro P", "initials": "PP"}, {"family": "Park", "given": "Sun-Mi", "initials": "SM"}, {"family": "Vergani", "given": "Stefano", "initials": "S"}, {"family": "Kharas", "given": "Michael G", "initials": "MG"}, {"family": "Yuan", "given": "Joan", "initials": "J"}, {"family": "Lahl", "given": "Katharina", "initials": "K"}, {"family": "Rosa", "given": "F\u00e1bio F", "initials": "FF"}, {"family": "Pires", "given": "Cristiana F", "initials": "CF"}, {"family": "Pereira", "given": "Carlos-Filipe", "initials": "CF"}], "type": "journal article", "published": "2025-10-14", "journal": {"title": "Immunity", "issn": "1097-4180", "volume": "58", "issue": "10", "pages": "2419-2438.e13", "issn-l": null}, "abstract": "Transcription factor cooperation is essential for specifying the heterogeneous dendritic cell (DC) lineages that orchestrate adaptive immunity, yet how it drives subset diversification remains poorly understood. Here, we employed a sequential anchored screen of 70 transcription factors using direct cellular reprogramming to identify regulators that specify type 2 conventional DCs (cDC2s) and plasmacytoid DCs (pDCs). We identified PU.1, IRF4, and PRDM1 as inducers of a pro-inflammatory cDC2B-like fate and SPIB, IRF8, and IKZF2 as mediators of an immature lymphoid DC program. Transcriptomic profiling linked these triads to lineage-specific signatures and demonstrated their requirement for subset identity. Mechanistically, lineage divergence was driven by chromatin co-engagement at subset-specific sites early in reprogramming. Functionally, reprogrammed DCs employed distinct immune mechanisms to elicit orthogonal anti-tumor responses in different tumor models. Collectively, our findings uncover transcriptional circuits that control DC diversification and pave the way to generate patient-tailored DC subsets for cancer immunotherapy.", "doi": "10.1016/j.immuni.2025.08.001", "pmid": "40885192", "labels": [], "xrefs": [{"db": "pii", "key": "S1074-7613(25)00335-8"}], "notes": [], "created": "2026-10-01T08:24:57.518Z", "modified": "2026-10-01T08:24:57.547Z"}