{"entity": "publication", "iuid": "24a800f4d198405e91995b986064482a", "timestamp": "2026-08-25T09:38:28.881Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/24a800f4d198405e91995b986064482a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/24a800f4d198405e91995b986064482a"}}, "title": "Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production.", "authors": [{"family": "Pinto", "given": "In\u00eas F", "initials": "IF", "orcid": "0000-0002-9714-4742", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/47785e08c3f84b7ebdcbf4b8b979ef21.json"}}, {"family": "Soares", "given": "Ruben R G", "initials": "RRG", "orcid": "0000-0001-5958-5232", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fef4d913e9e74505a000d8d8488cb62b.json"}}, {"family": "M\u00e4kinen", "given": "Meeri E-L", "initials": "ME"}, {"family": "Chotteau", "given": "Veronique", "initials": "V"}, {"family": "Russom", "given": "Aman", "initials": "A"}], "type": "journal article", "published": "2021-03-26", "journal": {"title": "ACS Sens", "issn": "2379-3694", "volume": "6", "issue": "3", "pages": "842-851", "issn-l": null}, "abstract": "The biopharmaceutical market has been rapidly growing in recent years, creating a highly competitive arena where R&D is critical to strike a balance between clinical safety and profitability. Toward process optimization, the recent development and adoption of new process analytical technologies (PAT) highlight the dynamic complexity of mammalian/human cell culture processes, as well as the importance of fine-tuning and modeling key metabolites and proteins. In this context, simple, rapid, and cost-effective devices allowing routine at-line monitoring of specific proteins during process development and production are currently lacking. Here, we report the development of a versatile microfluidic protein analysis cartridge allowing the multiplexed bead-based immunodetection of specific proteins directly from complex mixtures with minimal hands-on time. Colorimetric quantification of Chinese hamster ovary (CHO) host cell proteins as key impurities, monoclonal antibodies as target biopharmaceuticals, and lactate dehydrogenase as a marker of cell viability was achieved with limits of detection in the 1-10 ng/mL range and analysis times as short as 30 min. The device was further demonstrated for the monitoring of a Rituximab-producing CHO cell bioreactor over the course of 8 days, providing comparable recoveries to standard enzyme-linked immunosorbent assay (ELISA) kits. The high sensitivity combined with robustness to matrix interference highlights the potential of the device to perform at-line measurements spanning from the bioreactor to the downstream processing.", "doi": "10.1021/acssensors.0c01884", "pmid": "33724791", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8034812"}], "notes": [], "created": "2026-08-20T08:13:02.661Z", "modified": "2026-08-20T08:13:25.751Z"}