{"entity": "publication", "iuid": "22f63c8786994adcad826a7a62e7c618", "timestamp": "2026-08-23T09:27:26.404Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/22f63c8786994adcad826a7a62e7c618.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/22f63c8786994adcad826a7a62e7c618"}}, "title": "Click chemistry-based dual nanosystem for microRNA-122 detection with single-base specificity from tumour cells.", "authors": [{"family": "Robles-Remacho", "given": "Agust\u00edn", "initials": "A"}, {"family": "Martos-Jamai", "given": "Ismael", "initials": "I"}, {"family": "Tabraue-Ch\u00e1vez", "given": "Mavys", "initials": "M"}, {"family": "Aguilar-Gonz\u00e1lez", "given": "Araceli", "initials": "A"}, {"family": "Laz-Ruiz", "given": "Jose A", "initials": "JA"}, {"family": "Cano-Cort\u00e9s", "given": "M Victoria", "initials": "MV"}, {"family": "L\u00f3pez-Delgado", "given": "F Javier", "initials": "FJ"}, {"family": "Guardia-Monteagudo", "given": "Juan J", "initials": "JJ"}, {"family": "Pernagallo", "given": "Salvatore", "initials": "S"}, {"family": "Diaz-Mochon", "given": "Juan J", "initials": "JJ"}, {"family": "Sanchez-Martin", "given": "Rosario M", "initials": "RM"}], "type": "journal article", "published": "2024-12-23", "journal": {"title": "J Nanobiotechnology", "issn": "1477-3155", "volume": "22", "issue": "1", "pages": "791", "issn-l": null}, "abstract": "MicroRNAs (miRNAs) have been recognised as potential biomarkers due to their specific expression patterns in different biological tissues and their changes in expression under pathological conditions. MicroRNA-122 (miR-122) is a vertebrate-specific miRNA that is predominantly expressed in the liver and plays an important role in liver metabolism and development. Dysregulation of miR-122 expression is associated with several liver-related diseases, including hepatocellular carcinoma and drug-induced liver injury (DILI). Given the potential of miR-122 as a biomarker, its effective detection is important for accurate diagnosis. However, miRNA detection methods still face challenges, particularly in terms of accurately identifying miRNA isoforms that may differ by only a single base. Here, with the aim of advancing accessible methods for the detection of miRNAs with single-base specificity, we have developed a robust dual nanosystem that leverages the simplicity of click chemistry reactions. Using the dual nanosystem, we successfully detected miR-122 at single-base resolution using flow cytometry and analysed its expression in various tumour cell lines with high specificity and strong correlation with TaqMan assay results. We also detected miR-122 in serum and identified four single nucleotide variations in its sequence. The chemistry employed in this dual nanosystem is highly versatile and offers a promising opportunity to develop nanoparticle-based strategies that incorporate click chemistry and bioorthogonal chemistry for the detection of miRNAs and their isoforms.", "doi": "10.1186/s12951-024-03071-6", "pmid": "39710710", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11665063"}, {"db": "pii", "key": "10.1186/s12951-024-03071-6"}], "notes": [], "created": "2026-08-21T12:39:30.673Z", "modified": "2026-08-21T12:39:30.704Z"}