{"entity": "publication", "iuid": "21e3df77d7b74087ae563a842b36ae9a", "timestamp": "2026-05-21T02:26:34.104Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/21e3df77d7b74087ae563a842b36ae9a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/21e3df77d7b74087ae563a842b36ae9a"}}, "title": "Heterogeneity and interplay of the extracellular vesicle small RNA transcriptome and proteome.", "authors": [{"family": "Sork", "given": "Helena", "initials": "H"}, {"family": "Corso", "given": "Giulia", "initials": "G"}, {"family": "Krjutskov", "given": "Kaarel", "initials": "K"}, {"family": "Johansson", "given": "Henrik J", "initials": "HJ"}, {"family": "Nordin", "given": "Joel Z", "initials": "JZ"}, {"family": "Wiklander", "given": "Oscar P B", "initials": "OPB"}, {"family": "Lee", "given": "Yi Xin Fiona", "initials": "YXF"}, {"family": "Westholm", "given": "Jakub Orzechowski", "initials": "JO"}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J"}, {"family": "Wood", "given": "Matthew J A", "initials": "MJA"}, {"family": "M\u00e4ger", "given": "Imre", "initials": "I"}, {"family": "El Andaloussi", "given": "Samir", "initials": "S"}], "type": "journal article", "published": "2018-07-17", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "8", "issue": "1", "pages": "10813", "issn-l": "2045-2322"}, "abstract": "Extracellular vesicles (EVs) mediate cell-to-cell communication by delivering or displaying macromolecules to their recipient cells. While certain broad-spectrum EV effects reflect their protein cargo composition, others have been attributed to individual EV-loaded molecules such as specific miRNAs. In this work, we have investigated the contents of vesicular cargo using small RNA sequencing of cells and EVs from HEK293T, RD4, C2C12, Neuro2a and C17.2. The majority of RNA content in EVs (49-96%) corresponded to rRNA-, coding- and tRNA fragments, corroborating with our proteomic analysis of HEK293T and C2C12 EVs which showed an enrichment of ribosome and translation-related proteins. On the other hand, the overall proportion of vesicular small RNA was relatively low and variable (2-39%)\u00a0and mostly comprised of miRNAs and sequences mapping to piRNA loci. Importantly, this is one of the few studies, which systematically links vesicular RNA and protein cargo of\u00a0vesicles. Our data is particularly useful for future work in unravelling the biological mechanisms underlying vesicular RNA and protein sorting and serves\u00a0as an important guide in developing EVs as carriers for RNA therapeutics.", "doi": "10.1038/s41598-018-28485-9", "pmid": "30018314", "labels": {"Affiliated researcher": null}, "xrefs": [{"db": "pii", "key": "10.1038/s41598-018-28485-9"}, {"db": "pmc", "key": "PMC6050237"}], "notes": [], "created": "2019-01-17T13:55:12.049Z", "modified": "2019-01-17T13:55:12.068Z"}