{"entity": "publication", "iuid": "1d9e276d0f074c81b49252208fbe8ace", "timestamp": "2026-08-23T22:11:51.544Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/1d9e276d0f074c81b49252208fbe8ace.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/1d9e276d0f074c81b49252208fbe8ace"}}, "title": "Image-Based Quantification of Cell Debris as a Measure of Apoptosis.", "authors": [{"family": "\u00d6lander", "given": "Magnus", "initials": "M", "orcid": "0000-0002-4502-8184", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dacf28f90f084718817f085fed119acb.json"}}, {"family": "Handin", "given": "Niklas", "initials": "N"}, {"family": "Artursson", "given": "Per", "initials": "P"}], "type": "journal article", "published": "2019-05-07", "journal": {"title": "Anal. Chem.", "issn": "1520-6882", "volume": "91", "issue": "9", "pages": "5548-5552", "issn-l": "0003-2700"}, "abstract": "Apoptosis is a controlled form of cell death that can be induced by various diseases and exogenous toxicants. Common apoptosis-detection methods rely on fluorescent markers, which necessitate the use of costly reagents and time-consuming labeling procedures. Label-free methods avoid these problems, but often require specialized instruments instead. Here, we utilize apoptotic-cell disintegration to develop a novel label-free detection method based on the quantification of subcellular debris particles in bright-field-microscopy images. Debris counts show strong correlations with fluorescence-based annexin V staining and can be used to study concentration-dependent and temporal apoptosis activation. The method is rapid, low-cost, and easy to apply, as the only experimental step comprises bright-field imaging of culture-media samples followed by automated image processing. The late-stage nature of the debris measurement means that the method can complement other, established apoptosis assays, and its accessibility will allow a wider community of researchers to study apoptotic cell death.", "doi": "10.1021/acs.analchem.9b01243", "pmid": "31001971", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-21T11:32:40.187Z", "modified": "2026-08-21T11:32:40.247Z"}