{"entity": "publication", "iuid": "1cb9a60565604e1f9d6bb34b67615dbc", "timestamp": "2026-08-22T06:56:29.165Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/1cb9a60565604e1f9d6bb34b67615dbc.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/1cb9a60565604e1f9d6bb34b67615dbc"}}, "title": "AGPAT1 as a Novel Colonic Biomarker for Discriminating Between Ulcerative Colitis With and Without Primary Sclerosing Cholangitis.", "authors": [{"family": "Vessby", "given": "Johan", "initials": "J", "orcid": "0000-0003-1832-6386", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ed9e8d51dec4076855ed37e013bf22e.json"}}, {"family": "Wisniewski", "given": "Jacek R", "initials": "JR"}, {"family": "Lindskog", "given": "Cecilia", "initials": "C"}, {"family": "Eriksson", "given": "Niclas", "initials": "N"}, {"family": "Gabrysch", "given": "Katja", "initials": "K"}, {"family": "Zettl", "given": "Katharina", "initials": "K"}, {"family": "Wanders", "given": "Alkwin", "initials": "A"}, {"family": "Carlson", "given": "Marie", "initials": "M"}, {"family": "Rorsman", "given": "Fredrik", "initials": "F"}, {"family": "\u00c5berg", "given": "Mikael", "initials": "M"}], "type": "journal article", "published": "2022-05-01", "journal": {"title": "Clin Transl Gastroenterol", "issn": "2155-384X", "volume": "13", "issue": "5", "pages": "e00486", "issn-l": null}, "abstract": "Ulcerative colitis (UC) associated with primary sclerosing cholangitis (PSC-UC) is considered a unique inflammatory bowel disease (IBD) entity. PSC diagnosis in an IBD individual entails a significantly higher risk of gastrointestinal cancer; however, biomarkers for identifying patients with UC at risk for PSC are lacking. We, therefore, performed a thorough PSC-UC biomarker study, starting from archived colonic tissue.\n\nProteins were extracted out of formalin-fixed paraffin-embedded proximal colon samples from PSC-UC (n = 9), UC (n = 7), and healthy controls (n = 7). Patients with IBD were in clinical and histological remission, and all patients with UC had a history of pancolitis. Samples were processed by the multienzyme digestion FASP and subsequently analyzed by liquid chromatography-tandem mass spectrometry. Candidate proteins were replicated in an independent cohort (n: PSC-UC = 16 and UC = 21) and further validated by immunohistochemistry.\n\nIn the discovery step, 7,279 unique proteins were detected. The top 5 most differentiating proteins (PSC-UC vs UC) based on linear regression analysis were selected for replication. Of these, 1-acetylglycerol-3-phosphate O-acyltransferase 1 (AGPAT1) was verified as higher in PSC-UC than UC (P = 0.009) in the replication cohort. A difference on the group level was also confirmed by immunohistochemistry, showing more intense AGPAT1 staining in patients with PSC-UC compared with UC.\n\nWe present AGPAT1 as a potential colonic biomarker for differentiating PSC-UC from UC. Our findings have possible implication for future PSC-IBD diagnostics and surveillance.", "doi": "10.14309/ctg.0000000000000486", "pmid": "35363634", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9132532"}, {"db": "pii", "key": "01720094-202205000-00013"}], "notes": [], "created": "2026-08-21T12:53:06.775Z", "modified": "2026-08-21T12:53:06.815Z"}