{"entity": "publication", "iuid": "1b139bf7da254b1caadcb1a8ddff07fe", "timestamp": "2026-05-17T09:42:28.521Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/1b139bf7da254b1caadcb1a8ddff07fe.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/1b139bf7da254b1caadcb1a8ddff07fe"}}, "title": "Genome and physiology of the ascomycete filamentous fungus Xeromyces bisporus, the most xerophilic organism isolated to date.", "authors": [{"family": "Leong", "given": "Su-Lin L", "initials": "SL"}, {"family": "Lantz", "given": "Henrik", "initials": "H"}, {"family": "Pettersson", "given": "Olga V", "initials": "OV"}, {"family": "Frisvad", "given": "Jens C", "initials": "JC"}, {"family": "Thrane", "given": "Ulf", "initials": "U"}, {"family": "Heipieper", "given": "Hermann J", "initials": "HJ"}, {"family": "Dijksterhuis", "given": "Jan", "initials": "J"}, {"family": "Grabherr", "given": "Manfred", "initials": "M"}, {"family": "Pettersson", "given": "Mats", "initials": "M"}, {"family": "Tellgren-Roth", "given": "Christian", "initials": "C"}, {"family": "Schn\u00fcrer", "given": "Johan", "initials": "J"}], "type": "journal article", "published": "2015-02-00", "journal": {"title": "Environ. Microbiol.", "issn": "1462-2920", "volume": "17", "issue": "2", "pages": "496-513", "issn-l": "1462-2912"}, "abstract": "Xeromyces bisporus can grow on sugary substrates down to 0.61, an extremely low water activity. Its genome size is approximately 22\u2009Mb. Gene clusters encoding for secondary metabolites were conspicuously absent; secondary metabolites were not detected experimentally. Thus, in its 'dry' but nutrient-rich environment, X.\u2009bisporus appears to have relinquished abilities for combative interactions. Elements to sense/signal osmotic stress, e.g. HogA pathway, were present in X.\u2009bisporus. However, transcriptomes at optimal (\u223c\u20090.89) versus low aw (0.68) revealed differential expression of only a few stress-related genes; among these, certain (not all) steps for glycerol synthesis were upregulated. Xeromyces bisporus increased glycerol production during hypo- and hyper-osmotic stress, and much of its wet weight comprised water and rinsable solutes; leaked solutes may form a protective slime. Xeromyces bisporus and other food-borne moulds increased membrane fatty acid saturation as water activity decreased. Such modifications did not appear to be transcriptionally regulated in X.\u2009bisporus; however, genes modulating sterols, phospholipids and the cell wall were differentially expressed. Xeromyces bisporus was previously proposed to be a 'chaophile', preferring solutes that disorder biomolecular structures. Both X.\u2009bisporus and the closely related xerophile, Xerochrysium xerophilum, with low membrane unsaturation indices, could represent a phylogenetic cluster of 'chaophiles'. ", "doi": "10.1111/1462-2920.12596", "pmid": "25142400", "labels": {"Affiliated researcher": null}, "xrefs": [], "notes": [], "created": "2018-12-05T10:14:04.580Z", "modified": "2018-12-05T10:14:04.598Z"}