{"entity": "publication", "iuid": "1a75c4db536144a08e031373e01e4175", "timestamp": "2026-08-26T22:47:59.800Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/1a75c4db536144a08e031373e01e4175.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/1a75c4db536144a08e031373e01e4175"}}, "title": "Human proteins incorporated into tick-borne encephalitis virus revealed by in situ proximity ligation.", "authors": [{"family": "Ikebuchi", "given": "Ryoyo", "initials": "R"}, {"family": "Isaac", "given": "Alfred W", "initials": "AW"}, {"family": "Yoshii", "given": "Kentaro", "initials": "K"}, {"family": "Doulabi", "given": "Ehsan Manouchehri", "initials": "EM"}, {"family": "L\u00f6f", "given": "Liza", "initials": "L"}, {"family": "Azimi", "given": "Alireza", "initials": "A"}, {"family": "Chen", "given": "Lei", "initials": "L"}, {"family": "Fredolini", "given": "Claudia", "initials": "C"}, {"family": "Gallini", "given": "Radiosa", "initials": "R"}, {"family": "Landegren", "given": "Ulf", "initials": "U"}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M"}], "type": "journal article", "published": "2020-05-07", "journal": {"title": "Biochem. Biophys. Res. Commun.", "issn": "1090-2104", "volume": "525", "issue": "3", "pages": "714-719", "issn-l": "0006-291X"}, "abstract": "Host proteins incorporated into virus particles have been reported to contribute to infectivity and tissue-tropism. This incorporation of host proteins is expected to be variable among viral particles, however, protein analysis at single-virus levels has been challenging. We have developed a method to detect host proteins incorporated on the surface of virions using the in situ proximity ligation assay (isPLA) with rolling circle amplification (RCA), employing oligonucleotide-conjugated antibody pairs. The technique allows highly selective and sensitive antibody-based detection of viral and host proteins on the surface of individual virions. We detected recombinant noninfectious sub-viral particles (SVPs) of tick-borne encephalitis virus (TBEV) immobilized in microtiter wells as fluorescent particles detected by regular fluorescence microscopy. Counting the particles in the images enabled us to estimate individual TBEV-SVP counts in different samples. Using isPLA we detected individual calnexin-, CD9-, CD81-, CD29- and CD59-positive SVPs among the viral particles. Our data suggests that a diversity of host proteins may be incorporated into TEBV, illustrating that isPLA with digital counting enables single-virus analysis of host protein incorporation.", "doi": "10.1016/j.bbrc.2020.02.143", "pmid": "32139125", "labels": [], "xrefs": [{"db": "pii", "key": "S0006-291X(20)30432-0"}], "notes": [], "created": "2026-08-20T06:43:21.491Z", "modified": "2026-08-20T06:43:21.547Z"}