{"entity": "publication", "iuid": "07320ba1b1074f9ba0518f66a2c29632", "timestamp": "2026-09-03T05:16:57.474Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/07320ba1b1074f9ba0518f66a2c29632.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/07320ba1b1074f9ba0518f66a2c29632"}}, "title": "Benchmarking virus concentration methods for quantification of SARS-CoV-2 in raw wastewater.", "authors": [{"family": "Jafferali", "given": "Mohammed Hakim", "initials": "MH"}, {"family": "Khatami", "given": "Kasra", "initials": "K"}, {"family": "Atasoy", "given": "Merve", "initials": "M"}, {"family": "Birgersson", "given": "Madeleine", "initials": "M"}, {"family": "Williams", "given": "Cecilia", "initials": "C"}, {"family": "Cetecioglu", "given": "Zeynep", "initials": "Z"}], "type": "journal article", "published": "2021-02-10", "journal": {"title": "Sci. Total Environ.", "issn": "1879-1026", "volume": "755", "issue": "Pt 1", "pages": "142939", "issn-l": "0048-9697"}, "abstract": "Wastewater-based epidemiology offers a cost-effective alternative to testing large populations for SARS-CoV-2 virus, and may potentially be used as an early warning system for SARS-CoV-2 pandemic spread. However, viruses are highly diluted in wastewater, and a validated method for their concentration and further processing, and suitable reference viruses, are the main needs to be established for reliable SARS-CoV-2 municipal wastewater detection. For this purpose, we collected wastewater from two European cities during the Covid-19 pandemic and evaluated the sensitivity of RT-qPCR detection of viral RNA after four concentration methods (two variants of ultrafiltration-based method and two adsorption and extraction-based methods). Further, we evaluated one external (bovine corona virus) and one internal (pepper mild mottle virus) reference virus. We found a consistently higher recovery of spiked virus using the modified ultrafiltration-based method. This method also had a significantly higher efficiency (p-value <0.01) for wastewater SARS-CoV-2 detection. The ultracentrifugation method was the only method that detected SARS-CoV-2 in the wastewater of both cities. The pepper mild mottle virus was found to function as a potentially suitable internal reference standard.", "doi": "10.1016/j.scitotenv.2020.142939", "pmid": "33121776", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7553858"}, {"db": "pii", "key": "S0048-9697(20)36469-X"}], "notes": [], "created": "2026-08-20T08:04:34.902Z", "modified": "2026-08-20T08:04:34.915Z"}