{"entity": "journal", "iuid": "1a83d7c2de70448aa1d2c3b34c3fdf19", "timestamp": "2026-07-20T13:06:27.253Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/journal/One%20Health.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/journal/One%20Health"}}, "title": "One Health", "issn": "2352-7714", "issn-l": null, "publications_count": 2, "publications": [{"entity": "publication", "iuid": "ec28119bd1694596ac362b533bb1f623", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/ec28119bd1694596ac362b533bb1f623.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/ec28119bd1694596ac362b533bb1f623"}}, "title": "Emerging multi-drug resistant and extended-spectrum \u03b2-lactamase (ESBL)-positive enterotoxigenic E. coli (ETEC) clones circulating in aquatic environments and in patients.", "authors": [{"family": "Joffr\u00e9", "given": "Enrique", "initials": "E"}, {"family": "Mart\u00edn-Rodr\u00edguez", "given": "Alberto J", "initials": "AJ"}, {"family": "Justh de Neczpal", "given": "Annie", "initials": "A"}, {"family": "von Mentzer", "given": "Astrid", "initials": "A"}, {"family": "Sj\u00f6ling", "given": "\u00c5sa", "initials": "\u00c5"}], "type": "journal article", "published": "2025-06-00", "journal": {"title": "One Health", "issn": "2352-7714", "volume": "20", "pages": "100968", "issn-l": null}, "abstract": "Diarrheal disease pathogens often spread through water-borne routes. Enterotoxigenic Escherichia coli (ETEC) is a major bacterial agent causing diarrheal disease in children, adults, and travelers in endemic areas. In addition, ETEC is responsible for outbreaks of water and food-borne gasteroenteritis globally, ETEC isolates also show robust survival capacity in various environmental settings, including aquatic environments. During the last decade, studies of ETEC isolates have indicated a rapid increase in multi-drug resistant and extended-spectrum \u03b2-lactamase (ESBL)-positive human-specific ETEC strains. These have been found in both environmental water sources and human patients, warranting the urgent need for focused monitoring of antibiotic resistance development in ETEC. Whole genome sequencing (WGS) of isolates from environmental, animal, and human sources enables in silico surveillance of emerging pathogenic and multi-drug resistant strains. This method allows for re-analysis of genomic data, aiding in identification of new variants of pathogenic clones. By integrating data from diverse sources inclusing sequenced isolates, we found that certain ETEC clonal lineages e.g., those expressing certain toxin-colonization factor profiles including STp/CS6, LT STh/CS2 + CS3, and LT STh/CFA/I are more at risk to develop multi-drug resistance than other ETEC lineages. Comparizon of multi-locus sequence types from papers with WGS data indicated ST182, ST4, ST2332 and new ST types to be emerging multi-drug resistant ETEC. We conclude that further studies on sequenced ETEC/E. coli genomes are needed to enhance our understanding of the dynamics of ETEC evolution, and the relation of virulence and resistance profiles in both environmental and clinical isolates.", "doi": "10.1016/j.onehlt.2025.100968", "pmid": "39898314", "labels": {"Astrid von Mentzer": null, "DDLS Fellow": null}, "xrefs": [{"db": "pmc", "key": "PMC11786893"}, {"db": "pii", "key": "S2352-7714(25)00004-7"}], "notes": [], "created": "2025-12-02T15:47:00.162Z", "modified": "2025-12-02T15:47:00.164Z"}, {"entity": "publication", "iuid": "fd78ef4d1eb142daa15397e72f19853d", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/fd78ef4d1eb142daa15397e72f19853d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/fd78ef4d1eb142daa15397e72f19853d"}}, "title": "Fish are poor sentinels for surveillance of riverine antimicrobial resistance.", "authors": [{"family": "Tskhay", "given": "Faina", "initials": "F", "orcid": "0000-0001-8700-3279", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/66867423459544e7a72b0a9090a46cee.json"}}, {"family": "K\u00f6bsch", "given": "Christoph", "initials": "C", "orcid": "0000-0002-9748-2039", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ebe319eddda54827bf9ac3970e91a733.json"}}, {"family": "Elena", "given": "Alan X", "initials": "AX", "orcid": "0000-0001-5372-0923", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/005e2b93413c4627a222eb7a5ef66e04.json"}}, {"family": "Bengtsson-Palme", "given": "Johan", "initials": "J", "orcid": "0000-0002-6528-3158", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4c666068e9d34939b0252a627007cec7.json"}}, {"family": "Berendonk", "given": "Thomas U", "initials": "TU", "orcid": "0000-0002-9301-1803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4c66286f83a8430f954fbc5c52db10c5.json"}}, {"family": "Kl\u00fcmper", "given": "Uli", "initials": "U", "orcid": "0000-0002-4169-6548", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7d8605cdbfc14403b607a3b5ace7249d.json"}}], "type": "journal article", "published": "2025-06-00", "journal": {"title": "One Health", "issn": "2352-7714", "volume": "20", "pages": "101026", "issn-l": null}, "abstract": "Effective surveillance of antimicrobial resistance (AMR) in the environment is crucial for assessing the human and animal health risk of AMR pollution. Wastewater treatment plants (WWTPs) are one of the main sources of AMR pollutants discharged into water bodies. One important factor for assessing the risks associated with such pollution is the colonization potential of the resistant bacteria (ARB) and resistance genes (ARGs) from the environment into human or animal microbiomes upon exposure. This study explores whether fish can act as sentinels for surveillance of AMR pollution in general and specifically the human colonization potential of ARB in rivers impacted by WWTP effluents. Two riverine fish species, Brown trout, and European bullhead, were sampled up- and downstream a German WWTP. The two fish species were chosen due to their different lifestyles: Trout are mainly actively swimming in the water phase, while bullheads are sedentary and river sediment-associated. The bacterial microbiomes and resistomes of fish gills, skin, and feces were compared with those of the respective river water and sediment up- and downstream of the WWTP. Microbiomes of both fish mirrored the changes in river water and sediment downstream of the WWTP, with significant shifts in bacterial community composition, particularly an increase in Proteobacteria and Verrucomicrobia. However, increases in ARG abundances observed in water and sediment downstream of the WWTP were not reflected in any of the fish-associated resistomes. This indicates that while the fish microbiome is sensitive to environmental changes, resistomes of poikilothermic animals such as fish are less responsive to colonization by ARB originating from WWTPs and may not serve as effective sentinels for assessing AMR pollution and colonization risks in freshwater environments. This study highlights the complexity of using wildlife as indicators for environmental AMR pollution and suggests that other species are better suited for surveillance efforts.", "doi": "10.1016/j.onehlt.2025.101026", "pmid": "40236740", "labels": {"Johan Bengtsson-Palme": null, "DDLS Fellow": null}, "xrefs": [{"db": "pmc", "key": "PMC11999348"}, {"db": "pii", "key": "S2352-7714(25)00062-X"}], "notes": [], "created": "2025-06-04T08:12:42.896Z", "modified": "2025-09-09T09:29:29.395Z"}], "created": "2025-06-04T08:12:42.907Z", "modified": "2025-06-04T08:12:42.907Z"}