{"entity": "journal", "iuid": "f80bcac1ec7041d0a45172bdcd1770b7", "timestamp": "2026-08-29T04:15:51.693Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/journal/Oncol%20Rep.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/journal/Oncol%20Rep"}}, "title": "Oncol Rep", "issn": "1791-2431", "issn-l": null, "publications_count": 1, "publications": [{"entity": "publication", "iuid": "7e4992da61b541fd826c0f610e669af0", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7e4992da61b541fd826c0f610e669af0.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7e4992da61b541fd826c0f610e669af0"}}, "title": "Affibody\u2011mediated imaging of EGFR expression in prostate cancer using radiocobalt\u2011labeled DOTA\u2011ZEGFR:2377.", "authors": [{"family": "Mitran", "given": "Bogdan", "initials": "B"}, {"family": "Andersson", "given": "Ken G\u00f6sta", "initials": "KG"}, {"family": "Lindstr\u00f6m", "given": "Elin", "initials": "E"}, {"family": "Garousi", "given": "Javad", "initials": "J"}, {"family": "Rosestedt", "given": "Maria", "initials": "M"}, {"family": "Tolmachev", "given": "Vladimir", "initials": "V"}, {"family": "St\u00e5hl", "given": "Stefan", "initials": "S"}, {"family": "Orlova", "given": "Anna", "initials": "A"}, {"family": "L\u00f6fblom", "given": "John", "initials": "J"}], "type": "journal article", "published": "2019-01-00", "journal": {"title": "Oncol Rep", "issn": "1791-2431", "volume": "41", "issue": "1", "pages": "534-542", "issn-l": null}, "abstract": "The epidermal growth factor receptor (EGFR) is often overexpressed during prostate cancer (PCa) progression towards androgen\u2011independence after hormone therapy, but the overexpression is lower than in other types of cancers. Despite the low expression, EGFR has emerged as a promising therapeutic target for patients with castration\u2011resistant PCa. Non\u2011invasive methods for determination of EGFR expression in PCa can serve for patient stratification and therapy response monitoring. Radionuclide imaging probes based on affibody molecules (7 kDa) provide high contrast imaging of cancer\u2011associated molecular targets. We hypothesized that the anti\u2011EGFR affibody molecule DOTA\u2011ZEGFR:2377 labeled with 55Co (positron\u2011emitter, T1/2=17.5 h) would enable imaging of EGFR expression in PCa xenografts. The human PCa cell line DU\u2011145 was used for in vitro and in vivo experiments and 57Co was used as a surrogate for 55Co in the present study. Binding of 57Co\u2011DOTA\u2011ZEGFR:2377 to EGFR\u2011expressing xenografts was saturable with anti\u2011EGFR monoclonal antibody cetuximab, which would motivate the use of this tracer for monitoring the receptor occupancy during treatment. A signi\ufb01cant dose\u2011dependent difference in radioactivity accumulation in tumors and normal organs was observed when the biodistribution was studied 3 h after the injection of 10 and 35 \u00b5g of 57Co\u2011DOTA\u2011ZEGFR:2377: At lower doses the tumor uptake was 2\u2011fold higher although tumor\u2011to\u2011organ ratios were not altered. For clinically relevant organs for PCa, tumor\u2011to\u2011organ ratios increased with time, and at 24 h pi were 2.2\u00b10.5 for colon, 7\u00b12 for muscle, and 4.0\u00b10.7 for bones. Small animal SPECT/CT images confirmed the capacity of radiocobalt labeled DOTA\u2011ZEGFR:2377 to visualize EGFR expression in PCa. In conclusion, the present study demonstrated the feasibility of using the radiocobalt labeled anti\u2011EGFR affibody conjugate ZEGFR:2377 as an imaging agent for in vivo visualization of low EGFR\u2011expressing tumors, like PCa, and for monitoring of receptor occupancy during cetuximab therapy as well as the importance of optimal dosing in order to achieve higher sensitivity molecular imaging.", "doi": "10.3892/or.2018.6792", "pmid": "30320363", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-21T13:05:43.325Z", "modified": "2026-08-21T13:05:43.365Z"}], "created": "2026-08-21T13:05:43.333Z", "modified": "2026-08-21T13:05:43.333Z"}