{"entity": "journal", "iuid": "fddc4476f9104b2d9a013f720aa78169", "timestamp": "2026-09-23T21:35:18.872Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/journal/Neurooncol%20Adv.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/journal/Neurooncol%20Adv"}}, "title": "Neurooncol Adv", "issn": "2632-2498", "issn-l": null, "publications_count": 3, "publications": [{"entity": "publication", "iuid": "839b5dbb096a45369e05c4e2d36740ab", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/839b5dbb096a45369e05c4e2d36740ab.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/839b5dbb096a45369e05c4e2d36740ab"}}, "title": "Lack of ST2 aggravates glioma invasiveness, vascular abnormality, and immune suppression.", "authors": [{"family": "Wicher", "given": "Grzegorz", "initials": "G"}, {"family": "Roy", "given": "Ananya", "initials": "A"}, {"family": "Vaccaro", "given": "Alessandra", "initials": "A"}, {"family": "Vemuri", "given": "Kalyani", "initials": "K"}, {"family": "Ramachandran", "given": "Mohanraj", "initials": "M"}, {"family": "Olofsson", "given": "Tommie", "initials": "T"}, {"family": "Imbria", "given": "Rebeca-Noemi", "initials": "RN"}, {"family": "Belting", "given": "Mattias", "initials": "M"}, {"family": "Nilsson", "given": "Gunnar", "initials": "G"}, {"family": "Dimberg", "given": "Anna", "initials": "A"}, {"family": "Forsberg-Nilsson", "given": "Karin", "initials": "K", "orcid": "0000-0003-0692-6245", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6636b7d9d0e24ac2a15ccd0e9f631ed1.json"}}], "type": "journal article", "published": "2025-01-27", "journal": {"title": "Neurooncol Adv", "issn": "2632-2498", "volume": "7", "issue": "1", "pages": "vdaf010", "issn-l": null}, "abstract": "Glioblastoma (GBM) is the most common primary malignant brain tumor in adults, characterized by aggressive growth and a dismal prognosis. Interleukin-33 (IL-33) and its receptor ST2 have emerged as regulators of glioma growth, but their exact function in tumorigenesis has not been deciphered. Indeed, previous studies on IL-33 in cancer have yielded somewhat opposing results as to whether it is pro- or anti-tumorigenic.\n\nIL-33 expression was assessed in a GBM tissue microarray and public databases. As in vivo models we used orthotopic xenografts of patient-derived GBM cells, and syngenic models with grafted mouse glioma cells.\n\nWe analyzed the role of IL-33 and its receptor ST2 in nonmalignant cells of the glioma microenvironment and found that IL-33 levels are increased in cells surrounding the tumor. Protein complexes of IL-33 and ST2 are mainly found outside of the tumor core. The IL-33-producing cells consist primarily of oligodendrocytes. To determine the function of IL-33 in the tumor microenvironment, we used mice lacking the ST2 receptor. When glioma cells were grafted to ST2-deficient mouse brains, the resulting tumors exhibited a more invasive growth pattern, and are associated with poorer survival, compared to wild-type mice. Tumors in ST2-deficient hosts are more invasive, with increased expression of extracellular matrix remodeling enzymes and enhanced tumor angiogenesis. Furthermore, the absence of ST2 leads to a more immunosuppressive environment.\n\nOur findings reveal that glia-derived IL-33 and its receptor ST2 participate in modulating tumor invasiveness, tumor vasculature, and immunosuppression in glioma.", "doi": "10.1093/noajnl/vdaf010", "pmid": "39931535", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11808570"}, {"db": "pii", "key": "vdaf010"}], "notes": [], "created": "2026-09-23T13:22:38.560Z", "modified": "2026-09-23T13:22:38.613Z"}, {"entity": "publication", "iuid": "fabb8cc4033742df8dba5f2c6b5f8a32", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/fabb8cc4033742df8dba5f2c6b5f8a32.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/fabb8cc4033742df8dba5f2c6b5f8a32"}}, "title": "The potential of liquid biopsy for detection of the KIAA1549-BRAF fusion in circulating tumor DNA from children with pilocytic astrocytoma.", "authors": [{"family": "Krynina", "given": "Olha", "initials": "O", "orcid": "0009-0006-6911-557X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/517b17142f0a4e2692f81a14ab9958b2.json"}}, {"family": "de St\u00e5hl", "given": "Teresita D\u00edaz", "initials": "TD"}, {"family": "Jylh\u00e4", "given": "Cecilia", "initials": "C"}, {"family": "Arthur", "given": "Cecilia", "initials": "C"}, {"family": "Giraud", "given": "Geraldine", "initials": "G"}, {"family": "Nyman", "given": "Per", "initials": "P"}, {"family": "Fritzberg", "given": "Anders", "initials": "A"}, {"family": "Sandgren", "given": "Johanna", "initials": "J"}, {"family": "Tham", "given": "Emma", "initials": "E", "orcid": "0000-0001-6079-164X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3e5a7c4ae89f441ca0fa14238aa9511b.json"}}, {"family": "Sandvik", "given": "Ulrika", "initials": "U"}], "type": "journal article", "published": "2024-01-24", "journal": {"title": "Neurooncol Adv", "issn": "2632-2498", "volume": "6", "issue": "1", "pages": "vdae008", "issn-l": null}, "abstract": "Low-grade gliomas (LGGs) represent children's most prevalent central nervous system tumor, necessitating molecular profiling to diagnose and determine the most suitable treatment. Developing highly sensitive screening techniques for liquid biopsy samples is particularly beneficial, as it enables the early detection and molecular characterization of tumors with minimally invasive samples.\n\nWe examined CSF and plasma samples from patients with pilocytic astrocytoma (PA) using custom multiplexed droplet digital polymerase chain reaction (ddPCR) assays based on whole genome sequencing data. These assays included a screening test to analyze BRAF duplication and a targeted assay for the detection of patient-specific KIAA1549::BRAF fusion junction sequences or single nucleotide variants.\n\nOur findings revealed that 5 out of 13 individual cerebrospinal fluid (CSF) samples tested positive for circulating tumor DNA (ctDNA). Among these cases, 3 exhibited the KIAA1549::BRAF fusion, which was detected through copy number variation (CNV) analysis (n = 1) or a fusion-specific probe (n = 2), while 1 case each displayed the BRAF V600E mutation and the FGFR1 N577K mutation. Additionally, a quantitative analysis of cell-free DNA (cfDNA) concentrations in PA CSF samples showed that most cases had low cfDNA levels, below the limit of detection of our assay (<1.9 ng).\n\nWhile CNV analysis of CSF samples from LGGs still has some limitations, it has the potential to serve as a valuable complementary tool. Furthermore, it can also be multiplexed with other aberrations, for example, to the BRAF V600 test, to provide important insights into the molecular characteristics of LGGs.", "doi": "10.1093/noajnl/vdae008", "pmid": "38371226", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10874216"}, {"db": "pii", "key": "vdae008"}], "notes": [], "created": "2026-09-23T11:25:50.187Z", "modified": "2026-09-23T11:25:50.324Z"}, {"entity": "publication", "iuid": "8152f7c9aa8c44bb97fec9c3e843f07f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/8152f7c9aa8c44bb97fec9c3e843f07f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/8152f7c9aa8c44bb97fec9c3e843f07f"}}, "title": "Glioblastoma cytotoxicity conferred through dual disruption of endolysosomal homeostasis by Vacquinol-1.", "authors": [{"family": "Kwak", "given": "Dongoh", "initials": "D"}, {"family": "Hammarstr\u00f6m", "given": "Lars G J", "initials": "LGJ"}, {"family": "Haraldsson", "given": "Martin", "initials": "M"}, {"family": "Ernfors", "given": "Patrik", "initials": "P", "orcid": "0000-0002-1140-3986", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/473c11a7cdea464d8d7f2b30f7d8e8d9.json"}}], "type": "journal article", "published": "2021-10-15", "journal": {"title": "Neurooncol Adv", "issn": "2632-2498", "volume": "3", "issue": "1", "pages": "vdab152", "issn-l": null}, "abstract": "Increased membrane trafficking is observed in numerous cancer types, including glioblastoma. Targeting the oncogenic driven acquired alterations in membrane trafficking by synthetic cationic amphiphilic small molecules has recently been shown to induce death of glioblastoma cells, although the molecular targets are unknown.\n\nThe mechanism of action of the cationic amphiphilic drug Vacquinol-1 (Vacq1)-induced cytotoxicity was investigated using cell biology, biochemistry, functional experiments, chemical biology, unbiased antibody-based post-translation modification profiling, and mass spectrometry-based chemical proteomic analysis on patient-derived glioblastoma cells.\n\nVacq1 induced two types of abnormal endolysosomal vesicles, enlarged vacuoles and acidic vesicle organelles (AVOs). Mechanistically, enlarged vacuoles were formed by the impairment of lysosome reformation through the direct interaction and inhibition of calmodulin (CaM) by Vacq1, while AVO formation was induced by Vacq1 accumulation and acidification in the endosomal compartments through its activation of the v-ATPase. As a consequence of v-ATPase activation, cellular ATP consumption markedly increased, causing cellular energy shortage and cytotoxicity. This effect of Vacq1 was exacerbated by its inhibitory effects on calmodulin, causing lysosomal depletion and a failure of acidic vesicle organelle clearance.\n\nOur study identifies the targets of Vacq1 and the mechanisms underlying its selective cytotoxicity in glioblastoma cells. The dual function of Vacq1 sets in motion a glioblastoma-specific vicious cycle of ATP consumption resulting in cellular energy crisis and cell death.", "doi": "10.1093/noajnl/vdab152", "pmid": "34765974", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8577523"}, {"db": "pii", "key": "vdab152"}], "notes": [], "created": "2026-09-23T13:39:46.977Z", "modified": "2026-09-23T13:39:47.034Z"}], "created": "2026-09-23T11:25:50.291Z", "modified": "2026-09-23T11:25:50.291Z"}