{"entity": "journal", "iuid": "2aeee5de2f594a3c8c38db9720451ff2", "timestamp": "2026-07-20T13:42:48.587Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/journal/Mol%20Pain.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/journal/Mol%20Pain"}}, "title": "Mol Pain", "issn": "1744-8069", "issn-l": "1744-8069", "publications_count": 2, "publications": [{"entity": "publication", "iuid": "6edd5db068ed4fcb8a61f5d85800d578", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/6edd5db068ed4fcb8a61f5d85800d578.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/6edd5db068ed4fcb8a61f5d85800d578"}}, "title": "G protein-gated inwardly rectifying potassium channel subunits 1 and 2 are down-regulated in rat dorsal root ganglion neurons and spinal cord after peripheral axotomy.", "authors": [{"family": "Lyu", "given": "Chuang", "initials": "C"}, {"family": "Mulder", "given": "Jan", "initials": "J"}, {"family": "Barde", "given": "Swapnali", "initials": "S"}, {"family": "Sahlholm", "given": "Kristoffer", "initials": "K"}, {"family": "Zeberg", "given": "Hugo", "initials": "H"}, {"family": "Nilsson", "given": "Johanna", "initials": "J"}, {"family": "\u00c5rhem", "given": "Peter", "initials": "P"}, {"family": "H\u00f6kfelt", "given": "Tomas", "initials": "T"}, {"family": "Fried", "given": "Kaj", "initials": "K"}, {"family": "Shi", "given": "Tie-Jun Sten", "initials": "TJ"}], "type": "journal article", "published": "2015-07-22", "journal": {"title": "Mol Pain", "issn": "1744-8069", "volume": "11", "issue": null, "pages": "44", "issn-l": "1744-8069"}, "abstract": "Increased nociceptive neuronal excitability underlies chronic pain conditions. Various ion channels, including sodium, calcium and potassium channels have pivotal roles in the control of neuronal excitability. The members of the family of G protein-gated inwardly rectifying potassium (GIRK) channels, GIRK1-4, have been implicated in modulating excitability. Here, we investigated the expression and distribution of GIRK1 and GIRK2 in normal and injured dorsal root ganglia (DRGs) and spinal cord of rats.\n\nWe found that ~70% of the DRG neurons expressed GIRK1, while only <10% expressed GIRK2. The neurochemical profiles of GIRK1- and GIRK2-immunoreactive neurons were characterized using the neuronal markers calcitonin gene-related peptide, isolectin-B4 and neurofilament-200, and the calcium-binding proteins calbindin D28k, calretinin, parvalbumin and secretagogin. Both GIRK subunits were expressed in DRG neurons with nociceptive characteristics. However, while GIRK1 was widely expressed in several sensory neuronal subtypes, GIRK2 was detected mainly in a group of small C-fiber neurons. In the spinal dorsal horn, GIRK1- and -2-positive cell bodies and processes were mainly observed in lamina II, but also in superficial and deeper layers. Abundant GIRK1-, but not GIRK2-like immunoreactivity, was found in the ventral horn (laminae VI-X). Fourteen days after axotomy, GIRK1 and GIRK2 were down-regulated in DRG neurons at the mRNA and protein levels. Both after axotomy and rhizotomy there was a reduction of GIRK1- and -2-positive processes in the dorsal horn, suggesting a presynaptic localization of these potassium channels. Furthermore, nerve ligation caused accumulation of both subunits on both sides of the lesion, providing evidence for anterograde and retrograde fast axonal transport.\n\nOur data support the hypothesis that reduced GIRK function is associated with increased neuronal excitability and causes sensory disturbances in post-injury conditions, including neuropathic pain.", "doi": "10.1186/s12990-015-0044-z", "pmid": "26199148", "labels": {"Affiliated researcher": null}, "xrefs": [{"db": "pii", "key": "10.1186/s12990-015-0044-z"}, {"db": "pmc", "key": "PMC4511542"}], "notes": [], "created": "2018-12-05T11:31:05.167Z", "modified": "2018-12-05T11:31:05.186Z"}, {"entity": "publication", "iuid": "e9d7f06dd7324227b53273140bd846df", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e9d7f06dd7324227b53273140bd846df.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e9d7f06dd7324227b53273140bd846df"}}, "title": "Secretagogin is expressed in sensory CGRP neurons and in spinal cord of mouse and complements other calcium-binding proteins, with a note on rat and human.", "authors": [{"family": "Shi", "given": "Tie-Jun Sten", "initials": "TJ"}, {"family": "Xiang", "given": "Qiong", "initials": "Q"}, {"family": "Zhang", "given": "Ming-Dong", "initials": "MD"}, {"family": "Tortoriello", "given": "Giuseppe", "initials": "G"}, {"family": "Hammarberg", "given": "Henrik", "initials": "H"}, {"family": "Mulder", "given": "Jan", "initials": "J"}, {"family": "Fried", "given": "Kaj", "initials": "K"}, {"family": "Wagner", "given": "Ludwig", "initials": "L"}, {"family": "Josephson", "given": "Anna", "initials": "A"}, {"family": "Uhl\u00e9n", "given": "Mathias", "initials": "M"}, {"family": "Harkany", "given": "Tibor", "initials": "T"}, {"family": "H\u00f6kfelt", "given": "Tomas", "initials": "T"}], "type": "journal article", "published": "2012-10-29", "journal": {"title": "Mol Pain", "issn": "1744-8069", "volume": "8", "issue": null, "pages": "80", "issn-l": "1744-8069"}, "abstract": "Secretagogin (Scgn), a member of the EF-hand calcium-binding protein (CaBP) superfamily, has recently been found in subsets of developing and adult neurons. Here, we have analyzed the expression of Scgn in dorsal root ganglia (DRGs) and trigeminal ganglia (TGs), and in spinal cord of mouse at the mRNA and protein levels, and in comparison to the well-known CaBPs, calbindin D-28k, parvalbumin and calretinin. Rat DRGs, TGs and spinal cord, as well as human DRGs and spinal cord were used to reveal phylogenetic variations.\n\nWe found Scgn mRNA expressed in mouse and human DRGs and in mouse ventral spinal cord. Our immunohistochemical data showed a complementary distribution of Scgn and the three CaBPs in mouse DRG neurons and spinal cord. Scgn was expressed in ~7% of all mouse DRG neuron profiles, mainly small ones and almost exclusively co-localized with calcitonin gene-related peptide (CGRP). This co-localization was also seen in human, but not in rat DRGs. Scgn could be detected in the mouse sciatic nerve and accumulated proximal to its constriction. In mouse spinal cord, Scgn-positive neuronal cell bodies and fibers were found in gray matter, especially in the dorsal horn, with particularly high concentrations of fibers in the superficial laminae, as well as in cell bodies in inner lamina II and in some other laminae. A dense Scgn-positive fiber network and some small cell bodies were also found in the superficial dorsal horn of humans. In the ventral horn, a small number of neurons were Scgn-positive in mouse but not rat, confirming mRNA distribution. Both in mouse and rat, a subset of TG neurons contained Scgn. Dorsal rhizotomy strongly reduced Scgn fiber staining in the dorsal horn. Peripheral axotomy did not clearly affect Scgn expression in DRGs, dorsal horn or ventral horn neurons in mouse.\n\nScgn is a CaBP expressed in a subpopulation of nociceptive DRG neurons and their processes in the dorsal horn of mouse, human and rat, the former two co-expressing CGRP, as well as in dorsal horn neurons in all three species. Functional implications of these findings include the cellular refinement of sensory information, in particular during the processing of pain.", "doi": "10.1186/1744-8069-8-80", "pmid": "23102406", "labels": {"Affiliated researcher": null}, "xrefs": [{"db": "pii", "key": "1744-8069-8-80"}, {"db": "pmc", "key": "PMC3560279"}], "notes": [], "created": "2018-12-05T09:43:03.758Z", "modified": "2018-12-05T09:43:03.789Z"}], "created": "2018-12-05T09:43:03.771Z", "modified": "2020-11-27T13:12:56.737Z"}