{"entity": "journal", "iuid": "cc72af1668444f68a1fb38174431589d", "timestamp": "2026-08-15T13:13:19.644Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/journal/J.%20Cell.%20Sci..json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/journal/J.%20Cell.%20Sci."}}, "title": "J. Cell. Sci.", "issn": "1477-9137", "issn-l": "0021-9533", "publications_count": 7, "publications": [{"entity": "publication", "iuid": "0de25be8391c4b48b5027a6ce1eacf11", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/0de25be8391c4b48b5027a6ce1eacf11.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/0de25be8391c4b48b5027a6ce1eacf11"}}, "title": "CCG-1423-derived compounds reduce global RNA synthesis and inhibit transcriptional responses.", "authors": [{"family": "Prajapati", "given": "Bina", "initials": "B"}, {"family": "Sokolova", "given": "Maria", "initials": "M"}, {"family": "Sidorenko", "given": "Ekaterina", "initials": "E"}, {"family": "Kyriacou", "given": "Mikael", "initials": "M"}, {"family": "Kyher\u00f6inen", "given": "Salla", "initials": "S"}, {"family": "Vihervaara", "given": "Anniina", "initials": "A", "orcid": "0000-0001-6256-4694", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/18141880ac7c4db3a6bc41f43ec03713.json"}}, {"family": "Vartiainen", "given": "Maria K", "initials": "MK", "orcid": "0000-0002-2017-0475", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/621897c9b10541df95d8462481eaf0d9.json"}}], "type": "journal article", "published": "2024-07-01", "journal": {"title": "J. Cell. Sci.", "issn": "1477-9137", "volume": "137", "issue": "13", "issn-l": "0021-9533"}, "abstract": "Myocardin-related transcription factors (MRTFs) are coactivators of serum response factor (SRF), and thereby regulate cytoskeletal gene expression in response to actin dynamics. MRTFs have also been implicated in transcription of heat shock protein (HSP)-encoding genes in fly ovaries, but the mechanisms remain unclear. Here, we demonstrate that, in mammalian cells, MRTFs are dispensable for gene induction of HSP-encoding genes. However, the widely used small-molecule inhibitors of the MRTF-SRF transcription pathway, derived from CCG-1423, also efficiently inhibit gene transcription of HSP-encoding genes in both fly and mammalian cells in the absence of MRTFs. Quantifying RNA synthesis and RNA polymerase distribution demonstrates that CCG-1423-derived compounds have a genome-wide effect on transcription. Indeed, tracking nascent transcription at nucleotide resolution reveals that CCG-1423-derived compounds reduce RNA polymerase II elongation, and severely dampen the transcriptional response to heat shock. The effects of CCG-1423-derived compounds therefore extend beyond the MRTF-SRF pathway into nascent transcription, opening novel opportunities for their use in transcription research.", "doi": "10.1242/jcs.261790", "pmid": "38841882", "labels": {"Anniina Vihervaara": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pii", "key": "361133"}], "notes": [], "created": "2024-11-28T11:00:16.838Z", "modified": "2024-11-28T11:00:16.883Z"}, {"entity": "publication", "iuid": "3a87900802a7448e857df7bf5e97c0cc", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3a87900802a7448e857df7bf5e97c0cc.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3a87900802a7448e857df7bf5e97c0cc"}}, "title": "Uniting diversity to create a more inclusive academic environment.", "authors": [{"family": "Delemotte", "given": "Lucie", "initials": "L"}, {"family": "Garcia", "given": "Sarahi L", "initials": "SL"}, {"family": "Rodr\u00edguez-Gij\u00f3n", "given": "Alejandro", "initials": "A"}, {"family": "Sezgin", "given": "Erdinc", "initials": "E"}, {"family": "Vihervaara", "given": "Anniina", "initials": "A"}], "type": "journal article", "published": "2022-04-01", "journal": {"title": "J. Cell. Sci.", "issn": "1477-9137", "volume": "135", "issue": "7", "issn-l": "0021-9533"}, "abstract": null, "doi": "10.1242/jcs.259977", "pmid": "35411385", "labels": {"SciLifeLab Fellow": null, "Anniina Vihervaara": null, "Erdinc Sezgin": null, "Lucie Delemotte": null, "Sarahi Garcia": null}, "xrefs": [{"db": "pii", "key": "275020"}], "notes": [], "created": "2022-12-01T12:17:49.166Z", "modified": "2023-05-15T12:36:34.852Z"}, {"entity": "publication", "iuid": "0273ed7479ed4c949da548e6c02fbe77", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/0273ed7479ed4c949da548e6c02fbe77.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/0273ed7479ed4c949da548e6c02fbe77"}}, "title": "Reconstitution of immune cell interactions in free-standing membranes.", "authors": [{"family": "Jenkins", "given": "Edward", "initials": "E"}, {"family": "Santos", "given": "Ana Mafalda", "initials": "AM"}, {"family": "O'Brien-Ball", "given": "Caitlin", "initials": "C"}, {"family": "Felce", "given": "James H", "initials": "JH"}, {"family": "Wilcock", "given": "Martin J", "initials": "MJ"}, {"family": "Hatherley", "given": "Deborah", "initials": "D"}, {"family": "Dustin", "given": "Michael L", "initials": "ML"}, {"family": "Davis", "given": "Simon J", "initials": "SJ"}, {"family": "Eggeling", "given": "Christian", "initials": "C"}, {"family": "Sezgin", "given": "Erdinc", "initials": "E"}], "type": "journal article", "published": "2018-10-02", "journal": {"title": "J. Cell. Sci.", "issn": "1477-9137", "volume": "132", "issue": "4", "issn-l": "0021-9533"}, "abstract": "The spatiotemporal regulation of signalling proteins at the contacts formed between immune cells and their targets determines how and when immune responses begin and end. Therapeutic control of immune responses therefore relies on thorough elucidation of the molecular processes occurring at these interfaces. However, the detailed investigation of each component's contribution to the formation and regulation of the contact is hampered by the complexities of cell composition and architecture. Moreover, the transient nature of these interactions creates additional challenges, especially in the use of advanced imaging technology. One approach that circumvents these problems is to establish in vitro systems that faithfully mimic immune cell interactions, but allow complexity to be 'dialled-in' as needed. Here, we present an in vitro system that makes use of synthetic vesicles that mimic important aspects of immune cell surfaces. Using this system, we began to explore the spatial distribution of signalling molecules (receptors, kinases and phosphatases) and how this changes during the initiation of signalling. The GUV/cell system presented here is expected to be widely applicable.", "doi": "10.1242/jcs.219709", "pmid": "30209137", "labels": {"Erdinc Sezgin": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pii", "key": "jcs.219709"}, {"db": "pmc", "key": "PMC6398472"}], "notes": [], "created": "2020-09-28T08:54:12.927Z", "modified": "2022-11-07T11:31:19.118Z"}, {"entity": "publication", "iuid": "3b7666ce0c7547b2ba1b9eba7b5c8521", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3b7666ce0c7547b2ba1b9eba7b5c8521.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3b7666ce0c7547b2ba1b9eba7b5c8521"}}, "title": "Switch-like PKA responses in the nucleus of striatal neurons.", "authors": [{"family": "Yapo", "given": "C\u00e9dric", "initials": "C"}, {"family": "Nair", "given": "Anu G", "initials": "AG"}, {"family": "Hellgren Kotaleski", "given": "Jeanette", "initials": "J"}, {"family": "Vincent", "given": "Pierre", "initials": "P"}, {"family": "Castro", "given": "Liliana R V", "initials": "LRV"}], "type": "journal article", "published": "2018-07-27", "journal": {"title": "J. Cell. Sci.", "issn": "1477-9137", "volume": "131", "issue": "14", "issn-l": "0021-9533"}, "abstract": "Although it is known that protein kinase A (PKA) in the nucleus regulates gene expression, the specificities of nuclear PKA signaling remain poorly understood. Here, we combined computational modeling and live-cell imaging of PKA-dependent phosphorylation in mouse brain slices to investigate how transient dopamine signals are translated into nuclear PKA activity in cortical pyramidal neurons and striatal medium spiny neurons. We observed that the nuclear PKA signal in striatal neurons featured an ultrasensitive responsiveness, associated with fast all-or-none responses, which is not consistent with the commonly accepted theory of a slow and passive diffusion of catalytic PKA in the nucleus. Our numerical model suggests that a positive feed-forward mechanism inhibiting nuclear phosphatase activity - possibly mediated by DARPP-32 (also known as PPP1R1B) - could be responsible for this non-linear pattern of nuclear PKA response, allowing for a better detection of the transient dopamine signals that are often associated with reward-mediated learning.", "doi": "10.1242/jcs.216556", "pmid": "29967033", "labels": {"Affiliated researcher": null}, "xrefs": [{"db": "pii", "key": "jcs.216556"}], "notes": [], "created": "2019-01-17T14:01:54.251Z", "modified": "2019-01-17T14:01:54.277Z"}, {"entity": "publication", "iuid": "667755c20398457c9cd661570f57d14f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/667755c20398457c9cd661570f57d14f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/667755c20398457c9cd661570f57d14f"}}, "title": "Sexual dimorphism in the width of the mouse synaptonemal complex.", "authors": [{"family": "Agostinho", "given": "Ana", "initials": "A"}, {"family": "Kouznetsova", "given": "Anna", "initials": "A"}, {"family": "Hern\u00e1ndez-Hern\u00e1ndez", "given": "Abrahan", "initials": "A"}, {"family": "Bernhem", "given": "Kristoffer", "initials": "K"}, {"family": "Blom", "given": "Hans", "initials": "H"}, {"family": "Brismar", "given": "Hjalmar", "initials": "H"}, {"family": "H\u00f6\u00f6g", "given": "Christer", "initials": "C"}], "type": "journal article", "published": "2018-03-06", "journal": {"title": "J. Cell. Sci.", "issn": "1477-9137", "volume": "131", "issue": "5", "issn-l": "0021-9533"}, "abstract": "Sexual dimorphism has been used to describe morphological differences between the sexes, but can be extended to any biologically related process that varies between males and females. The synaptonemal complex (SC) is a tripartite structure that connects homologous chromosomes in meiosis. Here, aided by super-resolution microscopy techniques, we show that the SC is subject to sexual dimorphism, in mouse germ cells. We have identified a significantly narrower SC in oocytes and have established that this difference does not arise from a different organization of the lateral elements nor from a different isoform of transverse filament protein SYCP1. Instead, we provide evidence for the existence of a narrower central element and a different integration site for the C-termini of SYCP1, in females. In addition to these female-specific features, we speculate that post-translation modifications affecting the SYCP1 coiled-coil region could render a more compact conformation, thus contributing to the narrower SC observed in females.", "doi": "10.1242/jcs.212548", "pmid": "29420300", "labels": {"Affiliated researcher": null}, "xrefs": [{"db": "pii", "key": "jcs.212548"}], "notes": [], "created": "2018-12-05T12:22:46.614Z", "modified": "2018-12-05T12:22:46.633Z"}, {"entity": "publication", "iuid": "4688d3210b7a48138881291ed973d418", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/4688d3210b7a48138881291ed973d418.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/4688d3210b7a48138881291ed973d418"}}, "title": "Peptide-independent stabilization of MHC class I molecules breaches cellular quality control.", "authors": [{"family": "Hein", "given": "Zeynep", "initials": "Z"}, {"family": "Uchtenhagen", "given": "Hannes", "initials": "H"}, {"family": "Abualrous", "given": "Esam Tolba", "initials": "ET"}, {"family": "Saini", "given": "Sunil Kumar", "initials": "SK"}, {"family": "Jan\u00dfen", "given": "Linda", "initials": "L"}, {"family": "Van Hateren", "given": "Andy", "initials": "A"}, {"family": "Wiek", "given": "Constanze", "initials": "C"}, {"family": "Hanenberg", "given": "Helmut", "initials": "H"}, {"family": "Momburg", "given": "Frank", "initials": "F"}, {"family": "Achour", "given": "Adnane", "initials": "A"}, {"family": "Elliott", "given": "Tim", "initials": "T"}, {"family": "Springer", "given": "Sebastian", "initials": "S"}, {"family": "Boulanger", "given": "Denise", "initials": "D"}], "type": "journal article", "published": "2014-07-01", "journal": {"title": "J. Cell. Sci.", "issn": "1477-9137", "volume": "127", "issue": "Pt 13", "pages": "2885-2897", "issn-l": "0021-9533"}, "abstract": "The intracellular trafficking of major histocompatibility complex class I (MHC-I) proteins is directed by three quality control mechanisms that test for their structural integrity, which is correlated to the binding of high-affinity antigenic peptide ligands. To investigate which molecular features of MHC-I these quality control mechanisms detect, we have followed the hypothesis that suboptimally loaded MHC-I molecules are characterized by their conformational mobility in the F-pocket region of the peptide-binding site. We have created a novel variant of an MHC-I protein, K(b)-Y84C, in which two \u03b1-helices in this region are linked by a disulfide bond that mimics the conformational and dynamic effects of bound high-affinity peptide. K(b)-Y84C shows a remarkable increase in the binding affinity to its light chain, beta-2 microglobulin (\u03b22m), and bypasses all three cellular quality control steps. Our data demonstrate (1) that coupling between peptide and \u03b22m binding to the MHC-I heavy chain is mediated by conformational dynamics; (2) that the folded conformation of MHC-I, supported by \u03b22m, plays a decisive role in passing the ER-to-cell-surface transport quality controls; and (3) that \u03b22m association is also tested by the cell surface quality control that leads to MHC-I endocytosis. ", "doi": "10.1242/jcs.145334", "pmid": "24806963", "labels": {"Affiliated researcher": null}, "xrefs": [{"db": "pii", "key": "jcs.145334"}], "notes": [], "created": "2018-12-05T10:07:02.706Z", "modified": "2018-12-05T10:07:02.738Z"}, {"entity": "publication", "iuid": "4f921cadc7f5496a9a153660caa61230", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/4f921cadc7f5496a9a153660caa61230.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/4f921cadc7f5496a9a153660caa61230"}}, "title": "HSF1 at a glance.", "authors": [{"family": "Vihervaara", "given": "Anniina", "initials": "A"}, {"family": "Sistonen", "given": "Lea", "initials": "L"}], "type": "journal article", "published": "2014-01-15", "journal": {"title": "J. Cell. Sci.", "issn": "1477-9137", "volume": "127", "issue": "Pt 2", "pages": "261-266", "issn-l": "0021-9533"}, "abstract": "Heat shock factor 1 (HSF1) is an evolutionarily highly conserved transcription factor that coordinates stress-induced transcription and directs versatile physiological processes in eukaryotes. The central position of HSF1 in cellular homeostasis has been well demonstrated, mainly through its strong effect in transactivating genes that encode heat shock proteins (HSPs). However, recent genome-wide studies have revealed that HSF1 is capable of reprogramming transcription more extensively than previously assumed; it is also involved in a multitude of processes in stressed and non-stressed cells. Consequently, the importance of HSF1 in fundamental physiological events, including metabolism, gametogenesis and aging, has become apparent and its significance in pathologies, such as cancer progression, is now evident. In this Cell Science at a Glance article, we highlight recent advances in the HSF1 field, discuss the organismal control over HSF1, and present the processes that are mediated by HSF1 in the context of cell type, cell-cycle phase, physiological condition and received stimuli.", "doi": "10.1242/jcs.132605", "pmid": "24421309", "labels": {"Anniina Vihervaara": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pii", "key": "127/2/261"}], "notes": [], "created": "2023-12-03T19:27:00.968Z", "modified": "2023-12-03T19:27:00.973Z"}], "created": "2018-12-05T10:07:02.720Z", "modified": "2020-11-27T13:12:54.510Z"}