{"entity": "journal", "iuid": "cead8fa2826341b0a0fe7d16052b9746", "timestamp": "2026-08-23T09:24:59.337Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/journal/J%20Nanobiotechnology.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/journal/J%20Nanobiotechnology"}}, "title": "J Nanobiotechnology", "issn": "1477-3155", "issn-l": null, "publications_count": 2, "publications": [{"entity": "publication", "iuid": "22f63c8786994adcad826a7a62e7c618", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/22f63c8786994adcad826a7a62e7c618.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/22f63c8786994adcad826a7a62e7c618"}}, "title": "Click chemistry-based dual nanosystem for microRNA-122 detection with single-base specificity from tumour cells.", "authors": [{"family": "Robles-Remacho", "given": "Agust\u00edn", "initials": "A"}, {"family": "Martos-Jamai", "given": "Ismael", "initials": "I"}, {"family": "Tabraue-Ch\u00e1vez", "given": "Mavys", "initials": "M"}, {"family": "Aguilar-Gonz\u00e1lez", "given": "Araceli", "initials": "A"}, {"family": "Laz-Ruiz", "given": "Jose A", "initials": "JA"}, {"family": "Cano-Cort\u00e9s", "given": "M Victoria", "initials": "MV"}, {"family": "L\u00f3pez-Delgado", "given": "F Javier", "initials": "FJ"}, {"family": "Guardia-Monteagudo", "given": "Juan J", "initials": "JJ"}, {"family": "Pernagallo", "given": "Salvatore", "initials": "S"}, {"family": "Diaz-Mochon", "given": "Juan J", "initials": "JJ"}, {"family": "Sanchez-Martin", "given": "Rosario M", "initials": "RM"}], "type": "journal article", "published": "2024-12-23", "journal": {"title": "J Nanobiotechnology", "issn": "1477-3155", "volume": "22", "issue": "1", "pages": "791", "issn-l": null}, "abstract": "MicroRNAs (miRNAs) have been recognised as potential biomarkers due to their specific expression patterns in different biological tissues and their changes in expression under pathological conditions. MicroRNA-122 (miR-122) is a vertebrate-specific miRNA that is predominantly expressed in the liver and plays an important role in liver metabolism and development. Dysregulation of miR-122 expression is associated with several liver-related diseases, including hepatocellular carcinoma and drug-induced liver injury (DILI). Given the potential of miR-122 as a biomarker, its effective detection is important for accurate diagnosis. However, miRNA detection methods still face challenges, particularly in terms of accurately identifying miRNA isoforms that may differ by only a single base. Here, with the aim of advancing accessible methods for the detection of miRNAs with single-base specificity, we have developed a robust dual nanosystem that leverages the simplicity of click chemistry reactions. Using the dual nanosystem, we successfully detected miR-122 at single-base resolution using flow cytometry and analysed its expression in various tumour cell lines with high specificity and strong correlation with TaqMan assay results. We also detected miR-122 in serum and identified four single nucleotide variations in its sequence. The chemistry employed in this dual nanosystem is highly versatile and offers a promising opportunity to develop nanoparticle-based strategies that incorporate click chemistry and bioorthogonal chemistry for the detection of miRNAs and their isoforms.", "doi": "10.1186/s12951-024-03071-6", "pmid": "39710710", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11665063"}, {"db": "pii", "key": "10.1186/s12951-024-03071-6"}], "notes": [], "created": "2026-08-21T12:39:30.673Z", "modified": "2026-08-21T12:39:30.704Z"}, {"entity": "publication", "iuid": "fce859c435a94f7e84ec6c34991ef0b4", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/fce859c435a94f7e84ec6c34991ef0b4.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/fce859c435a94f7e84ec6c34991ef0b4"}}, "title": "Elasto-inertial microfluidics for bacteria separation from whole blood for sepsis diagnostics.", "authors": [{"family": "Faridi", "given": "Muhammad Asim", "initials": "MA"}, {"family": "Ramachandraiah", "given": "Harisha", "initials": "H"}, {"family": "Banerjee", "given": "Indradumna", "initials": "I"}, {"family": "Ardabili", "given": "Sahar", "initials": "S"}, {"family": "Zelenin", "given": "Sergey", "initials": "S"}, {"family": "Russom", "given": "Aman", "initials": "A", "orcid": "0000-0002-0242-358X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8939625551d5409aac4593329e2a6578.json"}}], "type": "journal article", "published": "2017-01-04", "journal": {"title": "J Nanobiotechnology", "issn": "1477-3155", "volume": "15", "issue": "1", "pages": "3", "issn-l": null}, "abstract": "Bloodstream infections (BSI) remain a major challenge with high mortality rate, with an incidence that is increasing worldwide. Early treatment with appropriate therapy can reduce BSI-related morbidity and mortality. However, despite recent progress in molecular based assays, complex sample preparation steps have become critical roadblock for a greater expansion of molecular assays. Here, we report a size based, label-free, bacteria separation from whole blood using elasto-inertial microfluidics.\n\nIn elasto-inertial microfluidics, the viscoelastic flow enables size based migration of blood cells into a non-Newtonian solution, while smaller bacteria remain in the streamline of the blood sample entrance and can be separated. We first optimized the flow conditions using particles, and show continuous separation of 5 \u03bcm particles from 2 \u03bcm at a yield of 95% for 5 \u00b5m particle and 93% for 2 \u00b5m particles at respective outlets. Next, bacteria were continuously separated at an efficiency of 76% from undiluted whole blood sample.\n\nWe demonstrate separation of bacteria from undiluted while blood using elasto-inertial microfluidics. The label-free, passive bacteria preparation method has a great potential for downstream phenotypic and molecular analysis of bacteria.", "doi": "10.1186/s12951-016-0235-4", "pmid": "28052769", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC5210221"}, {"db": "pii", "key": "10.1186/s12951-016-0235-4"}], "notes": [], "created": "2026-08-20T12:20:45.098Z", "modified": "2026-08-20T12:20:45.199Z"}], "created": "2026-08-20T12:20:45.163Z", "modified": "2026-08-20T12:20:45.163Z"}