{"entity": "journal", "iuid": "a23e51b675824fd5aa4cb6b016fc236e", "timestamp": "2026-08-20T21:18:39.649Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/journal/J%20Extracell%20Vesicles.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/journal/J%20Extracell%20Vesicles"}}, "title": "J Extracell Vesicles", "issn": "2001-3078", "issn-l": null, "publications_count": 2, "publications": [{"entity": "publication", "iuid": "a1e8842df8314e1fab9d332f761d6b8c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/a1e8842df8314e1fab9d332f761d6b8c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/a1e8842df8314e1fab9d332f761d6b8c"}}, "title": "Beyond basic characterization and omics: Immunomodulatory roles of platelet-derived extracellular vesicles unveiled by functional testing.", "authors": [{"family": "Palviainen", "given": "Mari", "initials": "M", "orcid": "0000-0002-7105-3543", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3bc89cf67f1842fdb3dd0d4fdf80a328.json"}}, {"family": "Puutio", "given": "Johanna", "initials": "J", "orcid": "0000-0002-5519-7053", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4301cceee9f34549b7a6fef5393670bc.json"}}, {"family": "\u00d8stergaard", "given": "Rikke Halse", "initials": "RH", "orcid": "0000-0001-6341-3871", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6f06d9129e024ccfb508779f65409be5.json"}}, {"family": "Eble", "given": "Johannes A", "initials": "JA"}, {"family": "Maaninka", "given": "Katariina", "initials": "K", "orcid": "0000-0003-1211-3267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/069c55d42f5942918124cd1c45dc8a3e.json"}}, {"family": "Butt", "given": "Umar", "initials": "U", "orcid": "0000-0002-0696-9520", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3556274862cd4eba8a4829c467f39322.json"}}, {"family": "Ndika", "given": "Joseph", "initials": "J", "orcid": "0000-0003-2326-5821", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ee4cfe2ca7a9427797157a50650d66b2.json"}}, {"family": "Kari", "given": "Otto K", "initials": "OK", "orcid": "0000-0003-3133-0670", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c5b8dc4904a146028699385aa0c144ea.json"}}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M"}, {"family": "Kjaer-Sorensen", "given": "Kasper", "initials": "K"}, {"family": "Oxvig", "given": "Claus", "initials": "C"}, {"family": "Aransay", "given": "Ana M", "initials": "AM", "orcid": "0000-0002-8271-612X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c8a11379529b4769a5f8b5a989e99330.json"}}, {"family": "Falcon-Perez", "given": "Juan M", "initials": "JM"}, {"family": "Federico", "given": "Antonio", "initials": "A"}, {"family": "Greco", "given": "Dario", "initials": "D"}, {"family": "Laitinen", "given": "Saara", "initials": "S"}, {"family": "Hayashi", "given": "Yuya", "initials": "Y"}, {"family": "Siljander", "given": "Pia R-M", "initials": "PR"}], "type": "journal article", "published": "2024-10-00", "journal": {"title": "J Extracell Vesicles", "issn": "2001-3078", "volume": "13", "issue": "10", "pages": "e12513", "issn-l": null}, "abstract": "Renowned for their role in haemostasis and thrombosis, platelets are also increasingly recognized for their contribution in innate immunity, immunothrombosis and inflammatory diseases. Platelets express a wide range of receptors, which allows them to reach a variety of activation endpoints and grants them immunomodulatory functions. Activated platelets release extracellular vesicles (PEVs), whose formation and molecular cargo has been shown to depend on receptor-mediated activation and environmental cues. This study compared the immunomodulatory profiles of PEVs generated via activation of platelets by different receptors, glycoprotein VI, C-type lectin-like receptor 2 and combining all thrombin-collagen receptors. Functional assays in vivo in zebrafish and in vitro in human macrophages highlighted distinct homing and secretory responses triggered by the PEVs. In contrast, omics analyses of protein and miRNA cargo combined with physicochemical particle characterization found only subtle differences between the activated PEV types, which were insufficient to predict their different immunomodulatory functions. In contrast, constitutively released PEVs, formed in the absence of an exogenous activator, displayed a distinct immunomodulatory profile from the receptor-induced PEVs. Our findings underscore that PEVs are tunable through receptor-mediated activation. To truly comprehend their role(s) in mediating platelet functions among immune cells, conducting functional assays is imperative.", "doi": "10.1002/jev2.12513", "pmid": "39330919", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11428872"}], "notes": [], "created": "2026-08-20T06:34:07.974Z", "modified": "2026-08-20T06:34:08.354Z"}, {"entity": "publication", "iuid": "623871df9ac243d8a817b9d8f9b50fdc", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/623871df9ac243d8a817b9d8f9b50fdc.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/623871df9ac243d8a817b9d8f9b50fdc"}}, "title": "Extracellular Vesicle Capture by AnTibody of CHoice and Enzymatic Release (EV-CATCHER): A customizable purification assay designed for small-RNA biomarker identification and evaluation of circulating small-EVs.", "authors": [{"family": "Mitchell", "given": "Megan I", "initials": "MI", "orcid": "0000-0002-1202-155X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/87c65c9726654b22a899be623395908c.json"}}, {"family": "Ben-Dov", "given": "Iddo Z", "initials": "IZ"}, {"family": "Liu", "given": "Christina", "initials": "C"}, {"family": "Ye", "given": "Kenny", "initials": "K"}, {"family": "Chow", "given": "Kar", "initials": "K"}, {"family": "Kramer", "given": "Yael", "initials": "Y"}, {"family": "Gangadharan", "given": "Anju", "initials": "A"}, {"family": "Park", "given": "Steven", "initials": "S"}, {"family": "Fitzgerald", "given": "Sean", "initials": "S"}, {"family": "Ramnauth", "given": "Andrew", "initials": "A"}, {"family": "Perlin", "given": "David S", "initials": "DS"}, {"family": "Donato", "given": "Michele", "initials": "M"}, {"family": "Bhoy", "given": "Emily", "initials": "E"}, {"family": "Manouchehri Doulabi", "given": "Ehsan", "initials": "E"}, {"family": "Poulos", "given": "Michael", "initials": "M"}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M"}, {"family": "Loudig", "given": "Olivier", "initials": "O"}], "type": "journal article", "published": "2021-06-00", "journal": {"title": "J Extracell Vesicles", "issn": "2001-3078", "volume": "10", "issue": "8", "pages": "e12110", "issn-l": null}, "abstract": "Circulating nucleic acids, encapsulated within small extracellular vesicles (EVs), provide a remote cellular snapshot of biomarkers derived from diseased tissues, however selective isolation is critical. Current laboratory-based purification techniques rely on the physical properties of small-EVs rather than their inherited cellular fingerprints. We established a highly-selective purification assay, termed EV-CATCHER, initially designed for high-throughput analysis of low-abundance small-RNA cargos by next-generation sequencing. We demonstrated its selectivity by specifically isolating and sequencing small-RNAs from mouse small-EVs spiked into human plasma. Western blotting, nanoparticle tracking, and transmission electron microscopy were used to validate and quantify the capture and release of intact small-EVs. As proof-of-principle for sensitive detection of circulating miRNAs, we compared small-RNA sequencing data from a subset of small-EVs serum-purified with EV-CATCHER to data from whole serum, using samples from a small cohort of recently hospitalized Covid-19 patients. We identified and validated, only in small-EVs, hsa-miR-146a and hsa-miR-126-3p to be significantly downregulated with disease severity. Separately, using convalescent sera from recovered Covid-19 patients with high anti-spike IgG titers, we confirmed the neutralizing properties, against SARS-CoV-2 in vitro, of a subset of small-EVs serum-purified by EV-CATCHER, as initially observed with ultracentrifuged small-EVs. Altogether our data highlight the sensitivity and versatility of EV-CATCHER.", "doi": "10.1002/jev2.12110", "pmid": "34122779", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8173589"}, {"db": "pii", "key": "JEV212110"}], "notes": [], "created": "2026-08-20T06:34:05.937Z", "modified": "2026-08-20T06:34:06.049Z"}], "created": "2026-08-20T06:34:06.015Z", "modified": "2026-08-20T06:34:06.015Z"}