{"entity": "journal", "iuid": "62c02219a4f242e09c1147b4fd384d91", "timestamp": "2026-08-20T20:45:46.011Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/journal/Intensive%20Care%20Med%20Exp.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/journal/Intensive%20Care%20Med%20Exp"}}, "title": "Intensive Care Med Exp", "issn": "2197-425X", "issn-l": null, "publications_count": 2, "publications": [{"entity": "publication", "iuid": "579372bf21154d27b1e909234a71c7da", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/579372bf21154d27b1e909234a71c7da.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/579372bf21154d27b1e909234a71c7da"}}, "title": "Repeated positron emission tomography tracing neutrophil elastase in a porcine intensive-care sepsis model.", "authors": [{"family": "Wilske", "given": "Frida", "initials": "F", "orcid": "0000-0002-2633-4063", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f13710aa09684aa6bb5e4110acb369a4.json"}}, {"family": "Eriksson", "given": "Olof", "initials": "O"}, {"family": "Amini", "given": "Rose-Marie", "initials": "RM"}, {"family": "Estrada", "given": "Sergio", "initials": "S"}, {"family": "Janols", "given": "Helena", "initials": "H"}, {"family": "Khalil", "given": "Amina", "initials": "A"}, {"family": "Larsson", "given": "Anders", "initials": "A"}, {"family": "Lipcsey", "given": "Mikl\u00f3s", "initials": "M"}, {"family": "Mangsbo", "given": "Sara", "initials": "S"}, {"family": "Sigfridsson", "given": "Jonathan", "initials": "J"}, {"family": "Sj\u00f6lin", "given": "Jan", "initials": "J"}, {"family": "Skorup", "given": "Paul", "initials": "P"}, {"family": "Wall", "given": "Anders", "initials": "A"}, {"family": "Wilson", "given": "Viola", "initials": "V"}, {"family": "Castegren", "given": "Markus", "initials": "M"}, {"family": "Antoni", "given": "Gunnar", "initials": "G"}], "type": "journal article", "published": "2025-02-04", "journal": {"title": "Intensive Care Med Exp", "issn": "2197-425X", "volume": "13", "issue": "1", "pages": "14", "issn-l": null}, "abstract": "Neutrophil granulocytes are important parts of the defence against bacterial infections. Their action is a two-edged sword, the mediators killing the intruding bacteria are at the same time causing tissue damage. Neutrophil activation is part of the dysregulated immune response to infection defining sepsis and neutrophil elastase is one of the powerful proteases causing both effects and damage. Inhibition of neutrophil elastase has been tried in sepsis and ARDS, so far with inconclusive results.\n\nWe used positron emission tomography (PET) combined with computed tomography (CT) and the selective and specific neutrophil elastase inhibitor PET-tracer [11C]GW457427 ([11C]NES), in an intensive care unit porcine Escherichia coli sepsis model with the primary aim to visualise the biodistribution of neutrophil elastase in the initial acute phase of the septic reaction. Repeated PET-CT investigations were performed before and after induction of sepsis.\n\nAt baseline [11C]NES uptake was found in the bone marrow, spleen and liver. The uptake in the bone marrow was markedly increased two hours into the sepsis, whereas in spleen and liver the uptake was not as markedly changed compared to baseline. At 4 h after the sepsis induction [11C]NES in the bone marrow decreased while the uptake increased in the spleen, liver and lungs.\n\nThe neutrophil elastase PET-tracer [11C]NES is a novel and unique instrument to study the acute innate neutrophil immune response in sepsis and associated vital organ failure. We here present images and quantitative data of the neutrophil elastase distribution the first hours of acute experimental sepsis. Surprisingly, a pronounced increase of neutrophil elastase was found in the bone marrow 2 h into the sepsis reaction followed at 4 h by increase in the liver, spleen and lungs and a concomitant reduction of the tracer uptake in bone marrow.", "doi": "10.1186/s40635-025-00721-3", "pmid": "39904820", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11794750"}, {"db": "pii", "key": "10.1186/s40635-025-00721-3"}], "notes": [], "created": "2026-08-20T12:37:11.168Z", "modified": "2026-08-20T12:37:11.245Z"}, {"entity": "publication", "iuid": "31b5d33f8c594981893ce4bf81817df1", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/31b5d33f8c594981893ce4bf81817df1.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/31b5d33f8c594981893ce4bf81817df1"}}, "title": "Regional distribution of mechanical strain and macrophage-associated lung inflammation after ventilator-induced lung injury: an experimental study.", "authors": [{"family": "Liggieri", "given": "Francesco", "initials": "F"}, {"family": "Chiodaroli", "given": "Elena", "initials": "E"}, {"family": "Pellegrini", "given": "Mariangela", "initials": "M"}, {"family": "Puuvuori", "given": "Emmi", "initials": "E"}, {"family": "Sigfridsson", "given": "Jonathan", "initials": "J"}, {"family": "Velikyan", "given": "Irina", "initials": "I"}, {"family": "Chiumello", "given": "Davide", "initials": "D"}, {"family": "Ball", "given": "Lorenzo", "initials": "L"}, {"family": "Pelosi", "given": "Paolo", "initials": "P"}, {"family": "Stramaglia", "given": "Sebastiano", "initials": "S"}, {"family": "Antoni", "given": "Gunnar", "initials": "G"}, {"family": "Eriksson", "given": "Olof", "initials": "O"}, {"family": "Perchiazzi", "given": "Gaetano", "initials": "G", "orcid": "0000-0001-6834-6399", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d6669bcffd7c4d038d751b06ab08c5da.json"}}], "type": "journal article", "published": "2024-09-03", "journal": {"title": "Intensive Care Med Exp", "issn": "2197-425X", "volume": "12", "issue": "1", "pages": "77", "issn-l": null}, "abstract": "Alveolar macrophages activation to the pro-inflammatory phenotype M1 is pivotal in the pathophysiology of Ventilator-Induced Lung Injury (VILI). Increased lung strain is a known determinant of VILI, but a direct correspondence between regional lung strain and macrophagic activation remains unestablished. [68Ga]Ga-DOTA-TATE is a Positron Emission Tomography (PET) radiopharmaceutical with a high affinity for somatostatin receptor subtype 2 (SSTR2), which is overexpressed by pro-inflammatory-activated macrophages. Aim of the study was to determine, in a porcine model of VILI, whether mechanical strain correlates topographically with distribution of activated macrophages detected by [68Ga]Ga-DOTA-TATE uptake.\n\nSeven anesthetized pigs underwent VILI, while three served as control. Lung CT scans were acquired at incremental tidal volumes, simultaneously recording lung mechanics. [68Ga]Ga-DOTA-TATE was administered, followed by dynamic PET scans. Custom MatLab scripts generated voxel-by-voxel gas volume and strain maps from CT slices at para-diaphragmatic (Para-D) and mid-thoracic (Mid-T) levels. Analysis of regional Voxel-associated Normal Strain (VoStrain) and [68Ga]Ga-DOTA-TATE uptake was performed and a measure of the statistical correlation between these two variables was quantified using the linear mutual information (LMI) method.\n\nCompared to controls, the VILI group exhibited statistically significant higher VoStrain and Standardized Uptake Value Ratios (SUVR) both at Para-D and Mid-T levels. Both VoStrain and SUVR increased along the gravitational axis with an increment described by statistically different regression lines between VILI and healthy controls and reaching the peak in the dependent regions of the lung (for strain in VILI vs. control was at Para-D: 760 \u00b1 210 vs. 449 \u00b1 106; at Mid-T level 497 \u00b1 373 vs. 193 \u00b1 160; for SUVR, in VILI vs. control was at Para-D: 2.2 \u00b1 1.3 vs. 1.3 \u00b1 0.1; at Mid-T level 1.3 \u00b1 1.0 vs. 0.6 \u00b1 0.03). LMI in both Para-D and Mid-T was statistically significantly higher in VILI than in controls.\n\nIn this porcine model of VILI, we found a topographical correlation between lung strain and [68Ga]Ga-DOTA-TATE uptake at voxel level, suggesting that mechanical alteration and specific activation of inflammatory cells are strongly linked in VILI. This study represents the first voxel-by-voxel examination of this relationship in a multi-modal imaging analysis.", "doi": "10.1186/s40635-024-00663-2", "pmid": "39225817", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11371987"}, {"db": "pii", "key": "10.1186/s40635-024-00663-2"}], "notes": [], "created": "2026-08-20T12:37:09.322Z", "modified": "2026-08-20T12:37:09.417Z"}], "created": "2026-08-20T12:37:09.377Z", "modified": "2026-08-20T12:37:09.377Z"}