{"entity": "journal", "iuid": "412cfeaefd2542778a6ae664f6bdae9c", "timestamp": "2026-07-22T16:47:54.933Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/journal/Eur%20Biophys%20J.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/journal/Eur%20Biophys%20J"}}, "title": "Eur Biophys J", "issn": "1432-1017", "issn-l": "0175-7571", "publications_count": 2, "publications": [{"entity": "publication", "iuid": "967d06684bbe44d2b0823df7ac4c194e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/967d06684bbe44d2b0823df7ac4c194e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/967d06684bbe44d2b0823df7ac4c194e"}}, "title": "Understanding biological dynamics: following cells and molecules to track functions and mechanisms", "authors": [{"family": "Palamidessi", "given": "A", "initials": "A"}, {"family": "Testa", "given": "I", "initials": "I"}, {"family": "Frittoli", "given": "E", "initials": "E"}, {"family": "Barozzi", "given": "S", "initials": "S"}, {"family": "Garr\u00e8", "given": "M", "initials": "M"}, {"family": "Mazza", "given": "D", "initials": "D"}, {"family": "Di Fiore", "given": "P P", "initials": "PP"}, {"family": "Diaspro", "given": "A", "initials": "A"}, {"family": "Scita", "given": "G", "initials": "G"}, {"family": "Faretta", "given": "Mario", "initials": "M"}], "type": "journal-article", "published": "2010-05-00", "journal": {"volume": "39", "issn": "0175-7571", "issue": "6", "pages": "947-957", "title": "Eur Biophys J", "issn-l": null}, "abstract": "The dissection of the molecular circuitries at the base of cell life and the identification of their abnormal transformation during carcinogenesis rely on the characterization of biological phenotypes generated by targeted overexpression or deletion of gene products through genetic manipulation. Fluorescence microscopy provides a wide variety of tools to monitor cell life with minimal perturbations. The observation of living cells requires the selection of a correct balance between temporal, spatial and \"statistical\" resolution according to the process to be analyzed. In the following paper ad hoc developed optical tools for dynamical tracking from cellular to molecular resolution will be presented. Particular emphasis will be devoted to discuss how to exploit light-matter interaction to selectively target specific molecular species, understanding the relationships between their intracellular compartmentalization and function.", "doi": "10.1007/s00249-009-0461-x", "pmid": "19455321", "labels": {"Ilaria Testa": null, "SciLifeLab Fellow": null}, "xrefs": [], "notes": [], "created": "2018-12-03T14:25:19.163Z", "modified": "2022-11-07T11:32:31.719Z"}, {"entity": "publication", "iuid": "867a4796843548f99301ae0e06dfbe81", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/867a4796843548f99301ae0e06dfbe81.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/867a4796843548f99301ae0e06dfbe81"}}, "title": "Photoactivation of pa-GFP in 3D: optical tools for spatial confinement.", "authors": [{"family": "Testa", "given": "I", "initials": "I"}, {"family": "Garr\u00e8", "given": "M", "initials": "M"}, {"family": "Parazzoli", "given": "D", "initials": "D"}, {"family": "Barozzi", "given": "S", "initials": "S"}, {"family": "Ponzanelli", "given": "I", "initials": "I"}, {"family": "Mazza", "given": "D", "initials": "D"}, {"family": "Faretta", "given": "M", "initials": "M"}, {"family": "Diaspro", "given": "A", "initials": "A"}], "type": "journal article", "published": "2008-09-00", "journal": {"title": "Eur Biophys J", "issn": "0175-7571", "volume": "37", "issue": "7", "pages": "1219-1227", "issn-l": null}, "abstract": "Photoactivatable fluorescent proteins represent an innovative tool for the direct observation of time dependent macromolecular events in living systems. The possibility of switching on a selected and confined subset of the expressed target proteins allows to follow biological processes reaching high signal to noise ratios. In particular, use of non-linear interactions to bring the molecules in the activated fluorescent form make it possible to extend the advantages of photoactivation to events that requires 3D spatial localization. In this work, we show the possibility to realize confined activated volumes in living cells, by employing photoactivatable green fluorescent protein (paGFP) in two-photon microscopy. The analysis of the kinetics of two-photon paGFP activation in dependence of the wavelength, the laser intensity and the exposure time is provided. This study allowed to assess the optimal conditions to induce photoactivation in living samples and to track the behaviour of tagged histone H2B during cellular division. Furthermore we investigate paGFP photoactivation under evanescent wave illumination. Total internal reflection set-up has been used to selectively activate subresolved distribution of proteins localized in the basal membrane surroundings. These two photoactivation methods provide a suitable tool for many biological applications, combining subresolved surface and in-depth three-dimensionally confined investigations.", "doi": "10.1007/s00249-008-0317-9", "pmid": "18379772", "labels": {"Ilaria Testa": null, "SciLifeLab Fellow": null}, "xrefs": [], "notes": [], "created": "2020-09-29T13:01:21.182Z", "modified": "2022-11-07T11:32:31.754Z"}], "created": "2018-12-03T14:25:19.177Z", "modified": "2020-11-27T13:12:53.677Z"}