{"entity": "journal", "iuid": "17e77e39f4dc4327b88038212ac8e384", "timestamp": "2026-07-14T04:29:46.675Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/journal/Eukaryotic%20Cell.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/journal/Eukaryotic%20Cell"}}, "title": "Eukaryotic Cell", "issn": "1535-9778", "issn-l": "1535-9786", "publications_count": 2, "publications": [{"entity": "publication", "iuid": "95475e54eb2941298ae41f333f7a8734", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/95475e54eb2941298ae41f333f7a8734.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/95475e54eb2941298ae41f333f7a8734"}}, "title": "Endosymbiotic Gene Transfer in Tertiary Plastid-Containing Dinoflagellates", "authors": [{"family": "Burki", "given": "Fabien", "initials": "F"}, {"family": "Imanian", "given": "Behzad", "initials": "B"}, {"family": "Hehenberger", "given": "Elisabeth", "initials": "E"}, {"family": "Hirakawa", "given": "Yoshihisa", "initials": "Y"}, {"family": "Maruyama", "given": "Shinichiro", "initials": "S"}, {"family": "Keeling", "given": "Patrick J", "initials": "PJ"}], "type": "journal-article", "published": "2014-02-00", "journal": {"volume": "13", "issn": "1535-9778", "issue": "2", "pages": "246-255", "title": "Eukaryotic Cell", "issn-l": "1535-9786"}, "abstract": "Plastid establishment involves the transfer of endosymbiotic genes to the host nucleus, a process known as endosymbiotic gene transfer (EGT). Large amounts of EGT have been shown in several photosynthetic lineages but also in present-day plastid-lacking organisms, supporting the notion that endosymbiotic genes leave a substantial genetic footprint in the host nucleus. Yet the extent of this genetic relocation remains debated, largely because the long period that has passed since most plastids originated has erased many of the clues to how this process unfolded. Among the dinoflagellates, however, the ancestral peridinin-containing plastid has been replaced by tertiary plastids on several more recent occasions, giving us a less ancient window to examine plastid origins. In this study, we evaluated the endosymbiotic contribution to the host genome in two dinoflagellate lineages with tertiary plastids. We generated the first nuclear transcriptome data sets for the \"dinotoms,\" which harbor diatom-derived plastids, and analyzed these data in combination with the available transcriptomes for kareniaceans, which harbor haptophyte-derived plastids. We found low level of detectable EGT in both dinoflagellate lineages, with only 9 genes and 90 genes of possible tertiary endosymbiotic origin in dinotoms and kareniaceans, respectively, suggesting that tertiary endosymbioses did not heavily impact the host dinoflagellate genomes.", "doi": "10.1128/ec.00299-13", "pmid": "24297445", "labels": {"Fabien Burki": null, "SciLifeLab Fellow": null}, "xrefs": [], "notes": [], "created": "2018-12-03T14:33:42.520Z", "modified": "2022-11-07T11:31:56.710Z"}, {"entity": "publication", "iuid": "a0b937523dd044dcb3cd0e0757c89bd5", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/a0b937523dd044dcb3cd0e0757c89bd5.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/a0b937523dd044dcb3cd0e0757c89bd5"}}, "title": "Genome-Based Reconstruction of the Protein Import Machinery in the Secondary Plastid of a Chlorarachniophyte Alga", "authors": [{"family": "Hirakawa", "given": "Yoshihisa", "initials": "Y"}, {"family": "Burki", "given": "Fabien", "initials": "F"}, {"family": "Keeling", "given": "Patrick J", "initials": "PJ"}], "type": "journal-article", "published": "2012-03-00", "journal": {"volume": "11", "issn": "1535-9778", "issue": "3", "pages": "324-333", "title": "Eukaryotic Cell", "issn-l": "1535-9786"}, "abstract": "Most plastid proteins are encoded by their nuclear genomes and need to be targeted across multiple envelope membranes. In vascular plants, the translocons at the outer and inner envelope membranes of chloroplasts (TOC and TIC, respectively) facilitate transport across the two plastid membranes. In contrast, several algal groups harbor more complex plastids, the so-called secondary plastids, which are surrounded by three or four membranes, but the plastid protein import machinery (in particular, how proteins cross the membrane corresponding to the secondary endosymbiont plasma membrane) remains unexplored in many of these algae. To reconstruct the putative protein import machinery of a secondary plastid, we used the chlorarachniophyte alga Bigelowiella natans, whose plastid is bounded by four membranes and still possesses a relict nucleus of a green algal endosymbiont (the nucleomorph) in the intermembrane space. We identified nine homologs of plant-like TOC/TIC components in the recently sequenced B. natans nuclear genome, adding to the two that remain in the nucleomorph genome (B. natans TOC75 [BnTOC75] and BnTIC20). All of these proteins were predicted to be localized to the plastid and might function in the inner two membranes. We also show that the homologs of a protein, Der1, that is known to mediate transport across the second membrane in the several lineages with secondary plastids of red algal origin is not associated with plastid protein targeting in B. natans. How plastid proteins cross this membrane remains a mystery, but it is clear that the protein transport machinery of chlorarachniophyte plastids differs from that of red algal secondary plastids.", "doi": "10.1128/ec.05264-11", "pmid": "22267775", "labels": {"Fabien Burki": null, "SciLifeLab Fellow": null}, "xrefs": [], "notes": [], "created": "2018-12-03T14:30:51.018Z", "modified": "2022-11-07T11:31:56.801Z"}], "created": "2018-12-03T14:30:51.031Z", "modified": "2020-11-27T13:12:55.209Z"}