{"entity": "journal", "iuid": "d58d918c7b324ca6bec5bb8f085481b6", "timestamp": "2026-08-20T21:18:40.381Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/journal/Cell%20Mol%20Bioeng.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/journal/Cell%20Mol%20Bioeng"}}, "title": "Cell Mol Bioeng", "issn": "1865-5025", "issn-l": null, "publications_count": 2, "publications": [{"entity": "publication", "iuid": "7ff490b158194d58b60b2b19cdaae261", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7ff490b158194d58b60b2b19cdaae261.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7ff490b158194d58b60b2b19cdaae261"}}, "title": "Effect of the Addition Frequency of 5-Azacytidine in Both Micro- and Macroscale Cultures.", "authors": [{"family": "Kadekar", "given": "Sandeep", "initials": "S"}, {"family": "Barbe", "given": "Laurent", "initials": "L"}, {"family": "Stoddart", "given": "Martin", "initials": "M"}, {"family": "Varghese", "given": "Oommen P", "initials": "OP"}, {"family": "Tenje", "given": "Maria", "initials": "M"}, {"family": "Mestres", "given": "Gemma", "initials": "G", "orcid": "0000-0001-7462-4236", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9b6944549b4b4480af1b441cd4c70f56.json"}}], "type": "journal article", "published": "2021-02-00", "journal": {"title": "Cell Mol Bioeng", "issn": "1865-5025", "volume": "14", "issue": "1", "pages": "121-130", "issn-l": null}, "abstract": "Human mesenchymal stem cells (hMSCs) have a great clinical potential for tissue regeneration purposes due to its multilineage capability. Previous studies have reported that a single addition of 5-azacytidine (5-AzaC) causes the differentiation of hMSCs towards a myocardial lineage. The aim of this work was to evaluate the effect of 5-AzaC addition frequency on hMSCs priming (i.e., indicating an early genetic differentiation) using two culture environments.\n\nhMSCs were supplemented with 5-AzaC while cultured in well plates and in microfluidic chips. The impact of 5-AzaC concentration (10 and 20 \u03bcM) and addition frequency (once, daily or continuously), as well as of culture period (2 or 5 days) on the genetic upregulation of PPAR\u03b3 (adipocytes), PAX3 (myoblasts), SOX9 (chondrocytes) and RUNX2 (osteoblasts) was evaluated.\n\nDaily delivering 5-AzaC caused a higher upregulation of PPAR\u03b3, SOX9 and RUNX2 in comparison to a single dose delivery, both under static well plates and dynamic microfluidic cultures. A particularly high gene expression of PPAR\u03b3 (tenfold-change) could indicate priming of hMSCs towards adipocytes.\n\nBoth macro- and microscale cultures provided results with similar trends, where addition frequency of 5-AzaC was a crucial factor to upregulate several genes. Microfluidics technology was proven to be a suitable platform for the continuous delivery of a drug and could be used for screening purposes in tissue engineering research.", "doi": "10.1007/s12195-020-00654-9", "pmid": "33633814", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7878657"}, {"db": "pii", "key": "654"}], "notes": [], "created": "2026-08-20T06:40:37.743Z", "modified": "2026-08-20T06:40:37.836Z"}, {"entity": "publication", "iuid": "bda398a8713a4a8090b7784773e10ca4", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/bda398a8713a4a8090b7784773e10ca4.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/bda398a8713a4a8090b7784773e10ca4"}}, "title": "Molecular Imaging of a New Multimodal Microbubble for Adhesion Molecule Targeting.", "authors": [{"family": "Ahmed", "given": "Mona", "initials": "M"}, {"family": "Gustafsson", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Aldi", "given": "Silvia", "initials": "S"}, {"family": "Dusart", "given": "Philip", "initials": "P"}, {"family": "Egri", "given": "Gabriella", "initials": "G"}, {"family": "Butler", "given": "Lynn M", "initials": "LM"}, {"family": "Bone", "given": "Dianna", "initials": "D"}, {"family": "D\u00e4hne", "given": "Lars", "initials": "L"}, {"family": "Hedin", "given": "Ulf", "initials": "U"}, {"family": "Caidahl", "given": "Kenneth", "initials": "K", "orcid": "0000-0002-5079-9696", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/848cce5f4b814e589d2e7fa26d98be72.json"}}], "type": "journal article", "published": "2019-02-00", "journal": {"title": "Cell Mol Bioeng", "issn": "1865-5025", "volume": "12", "issue": "1", "pages": "15-32", "issn-l": null}, "abstract": "Inflammation is an important risk-associated component of many diseases and can be diagnosed by molecular imaging of specific molecules. The aim of this study was to evaluate the possibility of targeting adhesion molecules on inflammation-activated endothelial cells and macrophages using an innovative multimodal polyvinyl alcohol-based microbubble (MB) contrast agent developed for diagnostic use in ultrasound, magnetic resonance, and nuclear imaging.\n\nWe assessed the binding efficiency of antibody-conjugated multimodal contrast to inflamed murine or human endothelial cells (ECs), and to peritoneal macrophages isolated from rats with peritonitis, utilizing the fluorescence characteristics of the MBs. Single-photon emission tomography (SPECT) was used to illustrate 99mTc-labeled MB targeting and distribution in an experimental in vivo model of inflammation.\n\nFlow cytometry and confocal microscopy showed that binding of antibody-targeted MBs to the adhesion molecules ICAM-1, VCAM-1, or E-selectin, expressed on cytokine-stimulated ECs, was up to sixfold higher for human and 12-fold higher for mouse ECs, compared with that of non-targeted MBs. Under flow conditions, both VCAM-1- and E-selectin-targeted MBs adhered more firmly to stimulated human ECs than to untreated cells, while VCAM-1-targeted MBs adhered best to stimulated murine ECs. SPECT imaging showed an approximate doubling of signal intensity from the abdomen of rats with peritonitis, compared with healthy controls, after injection of anti-ICAM-1-MBs.\n\nThis novel multilayer contrast agent can specifically target adhesion molecules expressed as a result of inflammatory stimuli in vitro, and has potential for use in disease-specific multimodal diagnostics in vivo using antibodies against targets of interest.", "doi": "10.1007/s12195-018-00562-z", "pmid": "31719897", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6816780"}, {"db": "pii", "key": "562"}], "notes": [], "created": "2026-08-20T06:40:35.583Z", "modified": "2026-08-20T06:40:35.706Z"}], "created": "2026-08-20T06:40:35.662Z", "modified": "2026-08-20T06:40:35.662Z"}